Fig. 4

A Schematic illustration of AuNPs for the specific enhancement of the immune response of NK cells towards HIV-infected T cells. B Representative confocal image of HIV-expressing cells coated with HIV gp120 recombinant protein (stained with PKH67 in green, and DAPI nuclei staining in blue) and NK cells (DAPI nuclei staining). Both cell types were co-cultured at 1:1 ratio for 20 min in the presence of the pBiAb-AuNPs (visualized in red). Scale bar = 5 μm. C Schematic synthesis process of the neutrophil-targeted Ab conjugated and drug or fluorescent-dye loaded PLGA-PEG NPs. D The forward scatter (FSC), side scatter (SSC) plots of PEGylated NPs (PNPs) or NIMP-R-14 Ab conjugated-PNPs (PINP) were analyzed by flow cytometer. The shift in FSC/SSC for PINPs indicated change in particle size due to NIMP-R-14 Ab conjugation (Left). The NIMP R-14 (FL2) positive neutrophils were isolated from PNP or PINP treated mice and analyzed for PINP delivery (FL1) to neutrophils. Flow data shows significantly higher delivery by PINP as compared to the PNP as negative control (Right). A, B Reproduced from [53], copyright permission by Elsevier 2020. C, D Reprinted from [54], copyright permission by Elsevier 2016