Fig. 4

Function of primary hepatocytes in electrospun PLGA scaffolds is improved with the addition of collagen 1. Scanning electron micrographs (SEM) of primary human hepatocytes within electrospun PLGA scaffolds after 14 days of culture in (a) unmodified PLGA, b PLGA with high [collagen], c PLGA with low [collagen], d PLGA with high [fibronectin] and e PLGA with low [fibronectin], scale bar = 50 μm. Insets: representative H&E stained hepatocytes within scaffolds, scale bar = 20 μm. f In vitro albumin secretion by hepatocytes cultured in PLGA scaffolds with/without collagen/fibronectin compared to sandwich culture. g In vitro urea synthesis of hepatocytes cultured in PLGA scaffolds with/without. Adapted with permission from Ref [201]