- Research article
- Open Access
3D printable hyaluronic acid-based hydrogel for its potential application as a bioink in tissue engineering
© The Author(s). 2019
- Received: 28 November 2018
- Accepted: 18 December 2018
- Published: 6 February 2019
After recognition of 3D printing and injectable hydrogel as a critical issue in tissue/organ engineering and regenerative medicine society, many hydrogels as bioinks have been developed worldwide by using polymeric biomaterials such as gelatin, alginate, hyaluronic acid and others. Even though some gels have shown good performances in 3D bioprinting, still their performances do not meet the requirements enough to be used as a bioink in tissue engineering.
In this study, a hydrogel consisting of three biocompatible biomaterials such as hyaluronic acid (HA), hydroxyethyl acrylate (HEA) and gelatin-methacryloyl, i.e. HA-g-pHEA-gelatin gel, has been evaluated for its possibility as a bioprinting gel, a bioink. Hydrogel synthesis was obtained by graft polymerization of HEA to HA and then grafting of gelatin- methacryloyl via radical polymerization mechanism. Physical and biological properties of the HA-based hydrogels fabricated with different concentrations of methacrylic anhydride (6 and 8%) for gelatin-methacryloylation have been evaluated such as swelling, rheology, morphology, cell compatibility, and delivery of small molecular dimethyloxalylglycine. Printings of HA-g-pHEA-Gelatin gel and its bioink with bone cell loaded in lattice forms were also evaluated by using home-built multi-material (3D bio-) printing system.
The experimental results demonstrated that the HA-g-pHEA-gelatin hydrogel showed both stable rheology properties and excellent biocompatibility, and the gel showed printability in good shape. The bone cells in bioinks of the lattice-printed scaffolds were viable. This study showed HA-g-pHEA-Gelatin gel’s potential as a bioink or its tissue engineering applications in injectable and 3D bioprinting forms.
- 3D bioprinting
- Hyaluronic acid
- Tissue engineering
3D Bioprinting has been recognized as one of the latest biotechnologies, which is highly used in tissue engineering and regenerative medicine to develop complex artificial tissue and organ structures to mimic native organs and tissues [1–7]. The bioprinting involves additive deposition of cells-loaded hydrogels in a predetermined structural architecture to regenerate functional and site-specific tissues or organs [4, 8]. This technique integrates hydrogels, live cells and controlled printing systems to create complex morphological structures, and has demonstrated precise control of the targeted structures than any currently available other methods [9–11]. Hence, very complex structures with controlled porosity, permeability and mechanical properties similar to patient’s own tissues and organs are possible by bioprinting [1, 2], with computer-aided design (CAD) and complex geometrical data such as magnetic resonance imaging (MRI), X-ray imaging and micro-computerized tomography scan (μ-CT-scan) . Even though there are many advantages of 3D bioprinting in biomedical field such as personalized patient-specific designs, high precision, on-demand creation of complex structures within a short time and with low cost, incorporation of cells in the printed scaffolds and hydrogels should be possible for tissue regeneration [5, 6].
Bioinks have recently attracted high interesting for development of functional tissues and organs in 3D bioprinting tissue engineering. Among bioink biomaterials, gelatin-methacrylates, agarose, alginate, collagen, chitin, silk, hyaluronic acid, cellulose and their mixtures have been employed as important bioink materials by using diverse cross-linking mechanisms such as click chemistry, ionic/hydrogen bonding in alginate bioinks and chemical bonding in alginate-methacrylate bioinks via radical initiators [12–21]. Alginate bioinks showed better cell encapsulation and survival, but their post-printing morphological stability is a critical issue to be resolved. Gelatin-methacrylate and modified collagen have been quickly obtained as bioinks by using cross-linking agents such as glutaldehyde, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), Eosin-Y and igarcure and etc. [22–25]. Even though morphological stability is excellent in this method, still removal of cross-linking agents and cytotoxicity should be solved in its final applications to patients. Furthermore, when applying the new bioink as in micro-extrusion printing, there are many huddles and challenges that needed to be overcome. Hyaluronic acid (HA) is progressively being applied for biomedical applications for decades since it is naturally biocompatible and indispensable in regulating cellular behaviors [24, 26, 27]. Again, though tissue functions of HA gel including cell migration, angiogenesis, viability and proliferation, its post-printing shape stability is weak, thus making its applications in bioprinting as bioinks.
The major challenges of bioinks are encapsulation of cells, bioprintability, biocompability, minimal cytotoxicity and high post-printing morphological stability, which maintains its shapes under wet condition to support cell adhesion and proliferation by modifying their chemical structures [28–30]. Herein, the purpose of our study was to evaluate the physical and biological properties of our newly developed hydrogel, as well as cell encapsulation in the gel. We recently reported a terpolymeric HA-HEA-PEGDA hydrogel to improve hydrogel’s mechanical stability by employing biocompatible polymers . To increase its cellular interaction with cells we adopted gelatin as a component of terpolymer gel, synthesizing a HA-HEA-gelatin hydrogel. Gelatin methacryloyl is an attractive photo-crsslinking polymer which is synthesized from chemical modification of gelatin with methacrylic anhydride. This terpolymeric HA-HEA-Gelatin hydrogel has been reported as our new work (31). In this study after evaluation of its diverse physical and biological properties such as swelling, drug release and rheological properties, we tested its printing and bioink printing ability to evaluate its potential possibility as bioinks by using home-built multi-material (3D bio-) printing system. The cells in bioprinted lattice scaffold were viable and the post-printed morphology was stable, indicating a possibility of its usage as a bioink.
Sodium salt of hyaluronic acid (HA, M.W. = 1660 kDa, PDI = 3.974) was graciously donated from Hanmi Pharm. Co. Ltd., Korea, and then chemically modified for gel synthesis [26, 30]. Potassium peroxodisulfate (KPS), gelatin (source: bovine skin), 2-hydroxyethyl acrylate (HEA), methacrylic anhydride (MA), dimethyloxaloylglycine (DMOG) and Dulbecco’s Modified Eagle Medium (DMEM) were purchased from Sigma Aldrich Chemical Co. (St. Luis, MO, USA, Germany and China). Tissue culture agents such as fetal bovine serum (FBS, Biotechnics Research, Mission Viejo, CA, USA), penicillin-streptomycin (Lonza, Seoul, Korea), 0.05% trypsin-EDTA-1X (Gibco-Life Technologies, Carlsbad, California, USA), and live & dead viability/cytotoxicity kit for mammalian cells (Invitrogen, Carlsbad, CA, USA) were purchased and used. Osteoblast precursor cell line derived from Mus musculus (mouse) calvaria, P9, was used for biocompatibility tests and distilled water (DW) was employed for all experiments.
Synthesis of gelatin-methacryloyl (gelatin-MA)
Synthesis of gelatin-methacrylation was performed by slight modification of the protocol described in the literature [29, 31]. At first, gelatin (1 g) was dissolved in 50 mL of phosphate buffer (pH 7.5) at 50 °C, and then methacrylic anhydride was added dropwise and stirred at 400 rpm. Different concentrations of methacrylic anhydride such as 4, 6 and 8% were employed to control its viscosity for printing. After 3 h, the reaction mixture was diluted with 50 mL of phosphate buffer solution (pH 7.5) and dialyzed for 4 days against distilled water at 40 °C for purification. The reaction product was freeze-dried and termed as Gelatin-MA in this study. The degree of substitution (DS) is determined by the method described in the literature and reported in our previous report .
Preparation of HA-based hydrogel
HA-based hydrogel was synthesized as below. Firstly, a homogeneous solution of HA (0.25 g, 0.623 × 10− 3 mol with respect to the molecular weight of one repeating unit) was added in 60 mL of distilled water into a 2-neck round bottom flask at room temperature. Next, the HA solution was located in a digital glass oil bath (LK Lab Korea, Korea) at 75 °C and stirred with a stirrer at 400 rpm. After 2 h, nitrogen gas was pursed into the solution for 30 min to make an inert atmosphere. After that, 5 mL aqueous KPS solution (0.0025 g, 0.0092 × 10− 3 mol) as initiator was mixed to the HA solution. After 20 min, 3 ml of HEA (17.41 × 10− 3 mol) as a monomer was poured to the mixture. When the viscosity of the solution changed, 5 mL aqueous Gelatin-MA solution (0.25 g) as a crosslinker was added and the reaction was processed for another 3 h, thus obtaining a gel-like product. Then, the gel-like product was purified by dialysis in distilled water at 25 °C for 2 days. The purified product (HA-g-pHEA-x-Gelatin-MA) was dried at lyophilizer at − 56 °C for 7 days, and used for characterizations and applications.
Morphological characterizations of hydrogel by digital and scanning electron microscope
After observation of hydrogel’s morphological images with digital camera, their morphological images of bioprinted hydrogel and bioinks, their images were taken by light microscopy (Olympus, Japan). The morphological images of hydrogels were also observed with SEM at different magnifications under inert environment after drying in − 78 C lyophilizer and then platinum coating for 1 min. The dry gel samples were fixed in advance on double sided tape on aluminum.
3D printing of HA-g-pHEA-gelatin hydrogels
Home-built multi-material (3D bio-) printing system (Seoul Tech) introduced in the previous paper  and equipped with rotating dual pressure-driven extruders and heating facilities was used to print the hydrogels. 3D gel structures with different templates and infills were designed using Solid works software (Dassault Systems SolidWorks Corp, USA), and the G-codes for the stereolithography (STL) files were generated using a slicing software (Simplify3D version 4.0, USA). The cross-linked HA-g-pHEA-Gelatin hydrogels (6 ml) were loaded inside the plastic syringe (10 ml, Musashi Engineering Inc., Korea) attached with a plastic orifice (25 gauge). The HA-g-pHEA-Gelatin gels in the syringe needle was placed proximal to the stage with the substrate by adjusting the Z-axis (syringe holder), X- and Y-axis (stage) using software. The pressure and temperature for printing were optimized by checking up the continuity and stability of the hydrogel extruded from the needle, and by varying other parameters such as printing pressure, temperature, nozzle and stage speed, and nozzle diameter. The optimized parameters were obtained as pressure 161 kPa, temperature 35 °C and speed 100 mm/min in our multi-material (3D bio) printing system. The HA-g-pHEA-Gelatin gels with and without MC3T3 cells were printed with 2 layers onto the glass coverslips (d = 1 cm). The live and dead images of the dispensed bone cells were acquired on day 3 after bioprinting, using a fluorescence microscope.
In vitro bone cell study
Behaviours of MC3T3 bone cells in the HA-g-pHEA-gelatin hydrogel
HA-g-pHEA-Gelatin hydrogel was sterilized by autoclave at 121 °C for 15 min, then placed in 24-well plates (300 μL/well). MC3T3 bone cells with low passage were in vitro cultured in DMEM media containing 10% fetal bovine serum and 1% penicillin-streptomycin in an incubator at the conditions of 37 °C and 5% CO2 atmosphere until getting confluence. After that, MC3T3 bone cells were trypsinized and injected into HA-g-pHEA-Gelatin gel (1 mL) at a density of 1 × 105 cells/wells. The bone cell-encapsulated hydrogels were cultured in 1 mL of DMEM (10% FBS and 1% penicillin-streptomycin). Culture medium was changed after 24 h of incubation and then after every 48 h. MC3T3 bone cells were also cultured on 24-well tissue culture plate (1 × 105 cells/wells) and used as a control.
Live & Dead assay
Cell viability on HA-g-pHEA-Gelatin hydrogel was evaluated by the live and dead assay after in vitro bone cell culture for 7 days. Live and dead viability/cytotoxicity assay for bone cells was processed according to the protocol suggested by the vendor (Invitrogen, USA). 1 mL of cell suspension was obtained from the HA-g-pHEA-Gelatin hydrogel. Two times of PBS washing was employed, and then the assay solutions that was composed of 1.2 μL of 2 mM ethidium homodimer-1 and 0.3 μL of 4 mM calcein AM (dead and live stains, respectively) in 600 μL PBS. In vitro cell viability in gel was observed by a fluorescence microscope (Leica DMLB, Germany) after 30 min incubation in 37 °C in the 5% CO2 incubator.
In vitro drug release study
Loading of DMOG in the HA-g-pHEA-gelatin gel
In vitro DMOG release study
In vitro DMOG release studies from small molecular DMOG-loaded HA-g-pHEA-Gelatin gel were performed at pH 7.4, and 37 °C. Briefly, the small molecular DMOG loaded gels were put in the flasks containing 20 mL of buffer media (pH 7.4). After 1, 3, 6, 24, and 48 h, aliquots were taken out from flasks and absorbance was measured by a UV-Vis spectrophotometer (Model: BioMATE 3, Thermo Scientific, Madison, USA). After each measurement, old buffer solutions were replaced by new buffer solutions. The % DMOG release were calculated on the basis of standard DMOG solutions. Each test was performed in triplicate.
All data were represented as mean ± standard deviation. Statistical significance was evaluated with one-way and multi-way ANOVA by using the SPSS 18.0 program (ver. 18.0, SPSS Inc., Chicago, IL, USA). The comparisons between two groups were performed by t-test, where, the significant level was p < 0.05.
Synthesis of HA-g-pHEA-gelatin gel
The HA-g-pHEA-Gelatin gel was synthesized using HA as a biopolymer, HEA as a monomer, Gelatin-MA (0.25 g) as a crosslinker, and KPS as an initiator at 75 °C as reported in previous. . In brief, sulphate anion radicals from KPS abstracted protons from hydroxyl groups of HA and then generated HA-macro-radicals. The reactive radicals of HA-g-pHEA reacts with methacrylate in Gelatin-MA. It was hypothesized that while all reactive sites were coupled with the one end of acrylamide site of Gelatin-MA, another site also connects another Gelatin-MA, thus acrylate group of Gelatin-MA took part in polymerization and formed a crosslinked network. The possible mechanism has been described in detail in a paper , by using the results of their chemical analyses such as 1H HR-MAS NMR, FTIR and TGA. We adopted this hydrogel with different concentrations of Gelatin-MA agents for the evaluations of both hydrogel and bioink its printability and in vitro cell viability in this study.
3D printability test and bioink printing of the HA-HEA-gelatin hydrogel
Bioinks and injectable hydrogels are considered as a key issue in tissue/organ engineering and regenerative medicine society, and many bioink hydrogels have been developed worldwide by using biocompatible polymers such as gelatin, agarose, chitosan, alginate, hyaluronic acid, silk, fibrin and other natural polymers . Biomaterial properties for bioinks include printability, mechanical/post-printing stability, controlled biodegradation, viscosity, modifiable functional side chain groups on the polymer. Biological requirements of bioinks include biocompatibility, which is not only non-toxic to the host tissues/cells, but also live cells’ viability inside bioink, cytocompatibility, and bioactivity of cells after bioprinting . Diverse crosslinking methods have been also reported for bioprinting such as photochemical crosslinking, ionic bonding, hydrophobic interactions, hydrogen bonding, self-crosslinking such as Diels-Elders reaction, Michael type reaction [3, 33].
Some hydrogels have demonstrated good performances in their applications in 3D bioprinting, but their performances do not meet the bioink requirements in tissue engineering. Even though alginate gel has self-associating ionic/hydrogen bonding during bioprinting, its post-printing stability and tissue regeneration performance are not good enough in its applications. Gelatin derivatives has been employed as bioprinting materials by many companies worldwide, but this method requires photochemical cross-linking agents, which have still biocompatibility issues. Mixture of poly (ethylene glycol) and silk with/without stem cells were reported as a self-standing bioink in 3D bioprinting and injectable gel for its application to cartilage regeneration [33, 34].
In this study, we evaluated a hydrogel for its potential application as a HA-g-pHEA-gelatin bioink, consisting of three biocompatible biomaterials such as HA, HEA and gelatin. This hydrogel was obtained by graft polymerization of HEA to HA and then grafting of gelatin-methacrylate via radical polymerization mechanism as reported in our previous paper. While HA has been reported as an important natural polymer for its applications to tissue regenerations such as cartilage, bone and blood vessel, gelatin has been employed for potentially higher cell adhesion and proliferation as a key polymeric component in terpolymer for tissue engineering. HEA has been employed as medical device polymers such as poly (hydroxyethyl acrylate). To utilize these properties of 3 components, we evaluated its potential as a bioink by expecting biocompatibility, mechanical properties by HEA and gelatin. Physical and biological properties of this hydrogel fabricated with different concentrations of methacrylic anhydride (6 and 8%) have demonstrated excellent properties such as good swelling, rheology, gel morphology, cyto-compatibility, and delivery of small molecular drug such as DMOG, even though there were no significant effects of its concentrations on their properties (4, 6 and 8%). These reasons have been reported to be the effects of saturation of methacrylate graft to gelatin . After verifying its physical and biological properties, HA-g-pHEA-Gelatin gel as bioink with bone cell loaded were bioprinted in lattice forms by using home-built multi-material (3D bio-) printing system. The experimental results demonstrated that the HA-g-pHEA-Gelatin hydrogel showed both stable rheology properties and excellent biocompatibility, and the gel showed printability in good shape.
The 3D printing of HA-g-pHEA-Gelatin hydrogel was successful and the bone cells in bioinks were viable, when printed in lattice forms. The three component hydrogel was biocompatible and gel printing processing was excellent. This study demonstrated the HA-g-pHEA-Gelatin gel has a potential to be used as a bioink or its tissue engineering applications.
We thank Eunchong Cha for her in vitro cell culture works and Dr. Dipankar Das for his help for Ms. Nahye Kim’s work.
This work was supported by the National Research Foundation of Korea (NRF) Grant (2015R1A2A1A10054592).
Availability of data and materials
All data generated and analyzed in this study are available from the corresponding author on request.
Nahye Kim synthesized all the hydrogels and analyzed their properties. Nahye Kim and Hao Nguen Hao printed all the hydrogels with and with cells loaded. Jaehoo Lee and professor Chibum Lee built up the 3D printing system. Professor Insup Noh supervised all the works and wrote all the manuscript. All authors read and approved the final manuscript.
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- Gopinathan J, Noh I. Recent trends on bioinks for 3D printing. Biomater Res. 2018;22(11), https://doi.org/10.1186/s40824-018-0122-1.
- Murphy SV, Atala A. 3D bioprinting of tissues and organs. Nature Biotech. 2014;32(8):773–85. https://doi.org/10.1038/nbt.2958.View ArticleGoogle Scholar
- Gopinathan J, Noh I. Click chemistry-based injectable hydrogels and bioprinting inks for tissue engineering applications. Tissue Eng and Regen Med. 2018;15(5):531–46.Google Scholar
- Gu BK, Choi DJ, Park SJ, Kim MS, Kang CM, Kim CH. 3-dimensional bioprinting for tissue engineering applications. Biomater Res. 2016;20(1):12. https://doi.org/10.1186/s40824-016-0058-2.View ArticleGoogle Scholar
- Chen C, Bang S, Cho Y, Lee S, Lee I, Zhang SM, Noh I. Research trends in biomimetic medical materials for tissue engineering: 3D bioprinting, surface modification, nano/micro-technology and clinical aspects in tissue engineering of cartilage and bone. Bioter Res. 2016;2016(20):11.Google Scholar
- Shafiee A, Atala A. Printing technologies for medical applications. Trends Mol Med. 2016;22(3):254–65. https://doi.org/10.1016/j.molmed.2016.01.003.View ArticleGoogle Scholar
- Guvendiren M, Molde J, Soares RM, Kohn J. Designing biomaterials for 3D printing. ACS Biomater Sci Eng. 2016;2(10):1679–93. https://doi.org/10.1021/acsbiomaterials.6b00121.View ArticleGoogle Scholar
- Hospodiuk M, Dey M, Sosnoski D, Ozbolat IT. The bioink: a comprehensive review on bioprintable materials. Biotechnol Adv. 2017. https://doi.org/10.1016/j.biotechadv.2016.12.006.
- Gudapati H, Dey M. Ozbolat I. a comprehensive review on droplet-based bioprinting: past, present and future. Biomaterials. 2016;102:20–42.View ArticleGoogle Scholar
- Nakamura M, Kobayashi A, Takagi F, Watanabe A, Hiruma Y, Ohuchi K, Iwasaki Y, Horie M, Morita I, Takatani S. Biocompatible inkjet printing technique for designed seeding of individual living cells. Tissue Eng. 2005;11(11–12):1658–66.View ArticleGoogle Scholar
- Mironov V, Boland T, Trusk T, Forgacs G, Markwald RR. Organ printing: computer-aided jet-based 3D tissue engineering. Trends Biotechnol. 2003;21(4):157–61.View ArticleGoogle Scholar
- Yi G, Son J, Yoo J, Park C, Koo H. Application of click chemistry in nanoparticle modification and its targeted delivery. Biomater Res. 2018;22:13.View ArticleGoogle Scholar
- Mao B, Divoux T, Snabre P. Impact of saccharides on the drying kinetics of agarose gels measured by in-situ interferometry. Sci Rep. 2017;7. https://doi.org/10.1038/srep41185.
- Zucca P, Fernandez-Lafuente R, Sanjust E. Agarose and its derivatives as supports for enzyme immobilization. Molecules. 2016;21(11):1577. https://doi.org/10.3390/molecules21111577.View ArticleGoogle Scholar
- Kreimendahl F, Köpf M, Thiebes AL, Duarte Campos DF, Blaeser A, Schmitz-Rode T, Apel C, Jockenhoevel S, Fischer H. Three-dimensional printing and angiogenesis: tailored agarose-type I collagen blends comprise three-dimensional printability and angiogenesis potential for tissue-engineered substitutes. Tissue Eng Part C: Methods. 2017;23(10):604–15.View ArticleGoogle Scholar
- Yang X, Lu Z, Wu H, Li W, Zheng L, Zhao J. Collagen-alginate as bioink for three-dimensional (3D) cell printing based cartilage tissue engineering. Mater Sci Eng C. 2018. https://doi.org/10.1016/j.msec.2017.09.002.
- Forget A, Blaeser A, Miessmer F, Köpf M, Campos DF, Voelcker NH, Blencowe A, Fischer H, Shastri VP. Mechanically tunable bioink for 3D bioprinting of human cells. Adv Healthcare Mater. 2017;6(20). https://doi.org/10.1002/adhm.201700255.
- Kulseng B, Skjåk-Bræk G, Ryan L, Andersson A, King A, Faxvaag A, Espevik T. Transplantation of alginate microcapsules: generation of antibodies against alginates and encapsulated porcine islet-like cell clusters. Transplantation. 1999;67(7):978–84.View ArticleGoogle Scholar
- Moradali MF, Ghods S, Rehm BH. Alginate biosynthesis and biotechnological production. In alginates and their biomedical applications 2018 (pp. 1–25). Springer, Singapore. doi: https://doi.org/10.1007/978-981-10-6910-9_1.
- Axpe E, Oyen ML. Applications of alginate-based bioinks in 3D bioprinting. Int J Mol Sci. 2016;17(12):1976.View ArticleGoogle Scholar
- Das D, Zhang S, Noh I. Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as biomaterials. Biomed Mater. 2017. https://doi.org/10.1088/1748-605X/aa8fa1.
- Jia W, Gungor-Ozkerim PS, Zhang YS, Yue K, Zhu K, Liu W, Pi Q, Byambaa B, Dokmeci MR, Shin SR, Khademhosseini A. Direct 3D bioprinting of perfusable vascular constructs using a blend bioink. Biomaterials. 2016;106:58–68.View ArticleGoogle Scholar
- Rodriguez-Pascual F, Slatter DA. Collagen cross-linking: insights on the evolution of metazoan extracellular matrix. Sci Rep. 2016;6.Google Scholar
- Stratesteffen H, Köpf M, Kreimendahl F, Blaeser A, Jockenhoevel S, Fischer H. GelMA-collagen blends enable drop-on-demand 3D printablility and promote angiogenesis. Biofabrication. 2017;9(4):045002.View ArticleGoogle Scholar
- Lee J, Yeo M, Kim W, Koo Y, Kim GH. Development of a tannic acid cross-linking process for obtaining 3D porous cell-laden collagen structure. Int J Biol Macromol. 2017. https://doi.org/10.1016/j.ijbiomac.2017.10.105.
- Das D, Pham TTH, Noh I. Characterization of hyaluronate-based terpolymeric hydrogel synthesized via radical polymerization mechanism for biomedical applications. Col Surface B Biointerface. 2018;170:64–75. https://doi.org/10.1016/j.colsurfb.2018.059.View ArticleGoogle Scholar
- Highley CB, Prestwich GD, Burdick JA. Recent advances in hyaluronic acid hydrogels for biomedical applications. Curr Opin Biotechnol. 2016;40:35–40.View ArticleGoogle Scholar
- Poldervaart MT, Goversen B, de Ruijter M, Abbadessa A, Melchels FP, Öner FC, Dhert WJ, Vermonden T, Alblas J. 3D bioprinting of methacrylated hyaluronic acid (MeHA) hydrogel with intrinsic osteogenicity. PLoS One. 2017;12(6):e0177628. https://doi.org/10.1371/journal.pone.0177628.View ArticleGoogle Scholar
- Sakai S, Ohi H, Hotta T, Kamei H, Taya M. Differentiation potential of human adipose stem cells bioprinted with hyaluronic acid/gelatin-based bioink through microextrusion and visible light-initiated crosslinking. Biopolymers. 2017. https://doi.org/10.1016/j.jmbbm.2017.09.031.
- Law N, Doney B, Glover H, Qin Y, Aman ZM, Sercombe TB, Liew LJ, Dilley RJ, Doyle BJ. Characterisation of hyaluronic acid methylcellulose hydrogels for 3D bioprinting. J Mech Behav Biomed Mater. 2018;77:389–99.View ArticleGoogle Scholar
- Das D, Cho H, Kim N, Pham TTH, Kim IG, Chung EJ, Noh I. A terpolymeric hydrogel of hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl with tunable properties as biomaterial. Carbohydr Polym. 2019;207:628–39.Google Scholar
- Lee J, Kim KE, Bang S, Noh I, Lee C. A desktop multi-material 3D bio-printing system with open-source hardware and software. Int J Precis Eng Manuf. 2017;18(4):605–12.View ArticleGoogle Scholar
- Davidson JR, Appuhamillage GA, Thompson CM, Voit W, Smaldone RA. Design paradigm utilizing reversible Diels–Alder reactions to enhance the mechanical properties of 3D printed materials. ACS Appl Mater Interfaces. 2016;8(26):16961–6. https://doi.org/10.1021/acsami.6b05118.View ArticleGoogle Scholar
- Zheng Z, Wu J, Liu M, Wang H, Li C, Rodriguez MJ, Li G, Wang X, Kaplan DL. 3D bioprinting of self-standing silk-based bioink. Adv Healthcare Mater. 2018;7:1701026.View ArticleGoogle Scholar
- Lee B.H., Lum N., Seow L.Y., Lim P.Q. and Tan L.P., Synthesis and characterization of types a and B gelatin methacryloyl for bioink applications, Materials 2016, 9, 797; doi:https://doi.org/10.3390/ma9100797.