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Comparison of in vivo antibacterial and antithrombotic activities of two types of pulmonary artery catheters in pig

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Abstract

Background

During pulmonary artery catheter (PAC) implantation, inaccurate measurements of hemodynamic parameters due to infection or thrombosis of PAC can result in severe complications.

Method

In order to develop a new PAC material, we evaluated the antibacterial and antithrombotic activities of the two types of PAC (Swan Ganz catheter and prototype catheter) in 14 pigs.

Results

In the 3-day group, bacterial infection rate was not different between the two types of PAC. In the 7-day group, bacterial infection rate of the prototype catheter was twice as elevated as that of the Swan-Ganz catheter. In the 3-day group, thrombus formation rate of the prototype catheter was twice as elevated as that of the Swan-Ganz catheter. In the 7-day group, thrombus formation rate was the same for the two types of PAC.

Conclusion

Here, we report an experimental pig model that confirms differences in antibacterial and antithrombotic activities.

Background

Pulmonary artery catheter (PAC) has been widely used in critical care since the 1970s. It is reported that 1.5 million PACs are used annually in the United States to monitor hemodynamic status of critically ill patients [1]. It plays an important role in assessing the patient’s condition in the intensive care unit or operating room, establishing a diagnosis of underlying pathology and deciding on the treatment plan by measuring hemodynamic parameters, such as cardiac output, mixed venous oxygen saturation and intra-cardiac pressures [2,3,4,5,6].

PAC consists of blood compatible catheter, temperature sensor and monitoring system. Biofilm formation due to infection or thrombosis near temperature sensor has been reported to cause serious problems [7,8,9]. As a result, the sensitivity of sensor was also reduced [10]. Currently, most cardiovascular medical devices are coated with lubricant, antithrombotic or antimicrobial agents. But there are no products that fundamentally solved serious side effects caused by prolonged implantation.

We compared the antibacterial and antithrombotic activities of the most commonly used PAC, Swan-Ganz catheter (CCCO Combo catheter: Vigilance II, Edwards® Lifesciences, California, USA), with those of the prototype catheter in a pig model in an effort to develop a new catheter material.

Materials and methods

Main difference between the two types of PAC is antithrombogenecity. Prototype catheter improved antithrombotic activity through a newly developed heparin coating procedure. Prototype catheter was composed of 72% Pellethane® 2363-55D (CAS # 37383–28-1, Compounding Solutions, Lewiston, Maine, USA), 20% Barium Sulfate (CAS # 7727–43-7, Compounding Solutions, Lewiston, Maine, USA) and 6% AD85H-M® (Antimicrobial agent, Compounding Solutions, Lewiston, Maine, USA). The shape of prototype catheter was similar to that of Swan-Ganz catheter.

Because several studies have documented that the cardiovascular system in pig is similar to that in humans, when compared with other animal models [11,12,13], we experimented with 14 female pigs. The subjects of this study were female Yorkshire Swine pigs weighing 45 ± 5 kg. The animals used in our experiments came from farms (XP bio® or Optipharm®, Cheongju, Korea) licensed by the Ministry of Food and Drug Safety, Republic of Korea. Pigs were moved from this farm to the Laboratory Animal Research Center Korea University College of Medicine 7 days before experiment and were housed in individual cages under controlled environments until experiment.

Bilateral external jugular veins were used to reduce errors due to individual difference (Fig. 1a). Experiments were conducted as follows. Prophylactic antibiotics (cefazolin 1 g, Chong Kun Dang®, Seoul, Korea) were injected intravenously 1 h before skin incision. General endotracheal anesthesia was induced with thiopental sodium 5–10 mg/kg and vecuronium bromide 0.1 mg/kg. Bilateral external jugular veins were exposed under sterile condition. Before jugular vein clamping, unfractionated heparin 50 IU/kg was injected intravenously. The fragment of catheter cut into 3 cm length was inserted into the vein via a tiny slit and were held to the vein with 6–0 prolene suture, which was used to close the venotomy (Fig. 1b). After hemostasis, the surgical wound was closed layer by layer. Low molecular weight heparin (LMWH, Clexane, Sanofi-Aventis®, Paris, France) at a dose of 1 mg/kg was injected subcutaneously every day until the catheters were removed. Three days later, 7 pigs underwent reoperation to remove the catheters. External jugular vein containing the catheter was resected into 4 cm length. We were careful not to disturb any thrombus that may have been present. The remaining 7 pigs underwent reoperation 7 days later. At the end of the operation, all pigs were euthanized by intravenous injection of potassium chloride 2 mEq/kg.

Fig. 1
figure1

Operative findings. a Exposure of both external jugular veins, b External jugular vein containing a catheter fragment

Results and discussion

Catheter tip culture was performed, and the culture was subsequently incubated for 48 h to evaluate antibacterial activity. The results are listed in Table 1. In the 3-day group, the bacterial infection rate was not different between the two types of PAC (42.86%). In the 7-day group, the bacterial infection rate of the prototype catheter (57.14%) was twice as elevated as that of the Swan-Ganz catheter (28.57%). Early in the experiment, the bacterial infection rate was as high as in the two types of PAC. The cultured bacteria were mainly normal flora in the gastrointestinal tract or the skin [14,15,16]. Therefore, contamination was suspected. After more carefully attending to sterilization and dressing, the bacterial infection rate decreased towards the end of the experiment.

Table 1 Comparison of bacterial Infection of the Swan-Ganz catheter and the prototype Catheter

Catheters implanted in pigs were isolated and examined under a microscope to determine the presence of thrombus.

The weight of thrombus was measured to evaluate antithrombotic activity from the 4th experiment. The results are listed in Table 2. In the 3-day group, the thrombus formation rate of the prototype catheter (85.71%) was twice as elevated as that of the Swan-Ganz catheter (42.85%). In the 7-day group, the thrombus formation rate was not different between the two types of PAC (71.43%). In the 3-day group, the mean thrombus amount of the prototype catheter (0.12 g) was six times as elevated as that of the Swan-Ganz catheter (0.02 g). In the 7-day group, the mean thrombus amount was 0.07 g in the prototype catheter and 0.12 g in the Swan-Ganz catheter. Despite anticoagulation therapy with LMWH, an increase in vulnerability to thrombus formation was observed in the PAC group, regardless of the type.

Table 2 Comparison of thrombus formation of the Swan-Ganz catheter and the prototype Catheter

Conclusion

In conclusion, after the 7-day implantation, there were differences in the rate of infection and thrombus formation between the Swan-Ganz catheter and the prototype catheter. The differences in these outcomes based on the material of the catheter.

Abbreviations

CFU:

Colony forming unit

E. avium:

Enterococcus avium

E. faecalis:

Enterococcus faecalis

LMWH:

Low molecular weight heparin

PAC:

Pulmonary artery catheter

S. aureus :

Staphylococcus aureus

S. chromogens:

Staphylococcus chromogens

S. epidermidis :

Staphylococcus epidermidis

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Acknowledgements

Not applicable

Funding

This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) & funded by the Korean government (MSIP & MOHW) (2015M3A9E2028644).

Availability of data and materials

All data generated or analyzed during this study are included in this published article.

Author information

JWH performed in vivo experiments and drafted the manuscript. YSS, JJK and HHS designed in vivo experiments and helped to draft the manuscript. All authors read and approved the final manuscript.

Correspondence to Ho Sung Son.

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Ethics approval

The Korea University College of Medicine Institutional Animal Care and Use Committee (IRB No. korea-2016-0118) approved this experiment protocol.

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All authors consent for publication.

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The authors declare that they have no competing interests.

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Han, J.W., Shin, Y.S., Kim, J.J. et al. Comparison of in vivo antibacterial and antithrombotic activities of two types of pulmonary artery catheters in pig. Biomater Res 21, 23 (2017) doi:10.1186/s40824-017-0109-3

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Keywords

  • Pulmonary artery catheter
  • Infection
  • Thrombosis
  • Pig