- Research article
- Open Access
Bioimaging of botulinum toxin and hyaluronate hydrogels using zwitterionic near-infrared fluorophores
© The Author(s). 2017
Received: 18 July 2017
Accepted: 11 September 2017
Published: 10 October 2017
The injection of botulinum toxin (BTX) to reduce facial wrinkles is one of the most frequently performed plastic surgery procedures. The biocompatible hydrogels are injected with BTX for effective tissue augmentation. However, it is difficult to determine the interval of injection for effective tissue augmentation.
BTX and hyaluronate (HA) hydrogels were labeled with zwitterionic (ZW) near-infrared (NIR) fluorophores and visualized for 3 weeks after injection to BALB/c nude mice.
BTX-ZW conjugates and diaminohexane (DAH)-HA-ZW hydrogels were successfully prepared by the conventional EDC/NHS chemistry. Using the NIR fluorescence imaging, we confirmed that approximately 10% of BTX-ZW conjugates and 50% of DAH-HA-ZW hydrogels remained 3 weeks post-injection.
This bioimaging technique using invisible NIR fluorescence light can be exploited for various biomedical applications.
Botulinum toxin (BTX), a neurotoxic protein derived from the bacterium Clostridium botulinum, is most frequently used for removing facial wrinkles [1, 2]. Small quantities of BTX can cause relaxation of overactive muscles and reduce wrinkles by smoothing overlying skin . BTX inhibits acetylcholine release and causes temporary chemical denervation at the neuromuscular junction by cleaving the synaptosomal-associated protein of 25 kDa [SNAP-25] on the internal surface of neuronal membranes, followed by vesicle fusion at the cellular level . After SNAP-25 regenerates over time, BTX effects diminish in the targeted muscles, and neuromuscular signaling and muscle contractility are restored .
Biocompatible hydrogels are generally injected with BTX to help fill skin wrinkles and effectively augment tissue volume [5, 6]. Among injectable dermal fillers, hyaluronate (HA) hydrogels have become especially popular for soft tissue augmentation since HA can absorb water to recover the volume of aging tissue [7–10]. HA fillers with a larger particle size and higher molecular weight are generally preferred to extend the duration under the skin, which can be obtained by crosslinking HA [11–13]. The typical dose of BTX is 200 unit in 3 months interval . The use of BTX and dermal fillers, however, threatens healthcare workers and patients due to residue left in the body, and it is a challenge to determine the dosing interval for effective tissue augmentation. Moreover, there have been previously reported the efficacy of Botox through bioimaging techniques [14, 15]. However, there were no studies to confirm the in vivo behavior of BTX.
Here, we firstly investigated in vivo dynamics of BTX and dermal fillers for tissue augmentation using near-infrared (NIR) fluorescence, which penetrates deeply into biological tissues . We previously reported that zwitterionic (ZW) NIR fluorophores have low serum binding, low nonspecific tissue uptake, and rapid elimination from the body through renal filtration . ZW fluorophores also have superior optical properties (i.e., high extinction coefficient and quantum yield) compared to visible dyes, which together improves quantitative bioimaging [18–21]. In this study, we investigate the in vivo dynamics of BTX and HA hydrogels for tissue augmentation by introducing ZW fluorophores in the chain.
Hyaluronate (HA) was purchased from Lifecore Co. (Chaska, MN). Botulinum toxins (BTX, Meditox, Ochang, Korea) were kindly gifted from Dr. Jeesoo An at the Wellman Center for Photomedicine in Massachusetts General Hospital. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was obtained from Tokyo Chemical Industry (Tokyo, Japan). N-hydroxysulfosuccinimide (sulfo-NHS), phosphate buffered saline (PBS), diaminohexane (DAH) and hydroxylbenzotriazole (HOBt) were purchased from Sigma (St. Louis, MO). Dialysis membrane tube was obtained from Thermo Scientific Co. (Waltham, MA).
Synthesis of Zwitterionic (ZW) NIR fluorophores
ZW NIR fluorophores were prepared as reported previously . In brief, Vilsmeier–Haack reagent was used for the condensation reaction with prepared intermediate indolium salts in anhydrous sodium acetate to prepare indocyanine based chloro-subtitued NIR fluorophore. And then, using microwave synthesis, a bifunctional phenoxypropionic acid linkage was introduced on the meso-chlorine atom to permit conjugation of targeting ligands. The crude product was washed against diethyl ether and precipitated in methanol and diethyl ether (20 mL, 1:4) to give the ZW NIR fluorophores.
Labeling of ZW fluorophores
HA and BTX were labeled with the amine modified ZW fluorophore, with emission wavelengths of 700 nm and 800 nm, respectively. HA (MW 100 kDa) was dissolved at a concentration of 5 mg/mL in double distilled water. After complete dissolution, ZW fluorophores (1 M ratio of HA) were added to the HA solution, and EDC and sulfo-NHS were added with 4 M ratio of HA to activate the carboxyl groups of HA. The pH of the reaction mixture was maintained at 6.5, and ZW fluorophore (1 M ratio of HA) was added to the solution and stirred overnight. After the reaction was stopped by changing the pH to 7.4, the resulting HA-ZW conjugate was purified with gel permeation chromatography (GPC) measuring the retention time. The mobile phase was PBS at pH 7.4 and the flow rate was 1 mL/min. The detection wavelength was 210 nm. The purified conjugate solution was lyophilized for 3 days. BTX was dissolved in phosphate buffered saline (PBS, Sigma, St. Louis, MO) and the conjugation and purification was performed as described above. The detection wavelength was 280 nm.
Preparation of DAH-HA-ZW hydrogels
DAH-HA-ZW hydrogel was prepared using the same method described elsewhere . HA-ZW conjugates were dissolved at a concentration of 30 mg/mL in double distilled water; after complete dissolution, DAH (1 M ratio of HA) was added to the HA solution for a cross-linking reaction with the carboxyl groups of HA. EDC and HOBt (1 M ratio of HA), activating the carboxyl groups of HA, were dissolved in water and added to the mixed solution of HA and DAH for DAH-HA hydrogel preparation. The final precursor solution was incubated at 37 °C for 2 h to complete the cross-linking reaction. Prepared DAH-HA-ZW hydrogels were sealed within dialysis membrane tube (MWCO of 7 kDa) and dialyzed against PBS for 3 days to remove the remaining EDC, HOBt, and DAH completely. The degree of modification in DAH-HA conjugates was analyzed by proton nuclear magnetic resonance (1H NMR, DRX-400, Bruker, Germany).
Bioimaging of BTX-ZW conjugates and DAH-HA-ZW hydrogels
BTX-ZW conjugates and DAH-HA-ZW hydrogel with the same fluorescence intensity were intramuscularly and subcutaneously inoculated into the BALB/c nude mice (100 pmol of ZW dye, 50 μL), sequentially. The home-built dual-channel imaging system  was used to acquire NIR fluorescent images at 0, 1, 2, and 3 weeks post-injection. Animals were housed in an AAALAC-certified facility and all animal studies were performed under the supervision of BIDMC IACUC in accordance with approved institutional protocol of #057–2014.
Results and discussion
Synthesis of BTX-ZW conjugates and DAH-HA-ZW hydrogel
Characterization of the BTX-ZW, HA-ZW, and DAH-HA conjugates
Dynamics of BTX-ZW conjugates and DAH-HA-ZW hydrogel
Bioimaging techniques using Zwitterionic (ZW) NIR fluorophores were successfully carried out to investigate in vivo dynamics of each component for tissue augmentation. BTX-ZW conjugates and DAH-HA-ZW hydrogel were synthesized, and in vivo dynamics were investigated using home-built bioimaging equipment. Bioimaging using ZW fluorophores for indication of bodily residue and the interval of agents can be exploited for various biomedical applications.
We thank Dr. Wonjin Park (W Beauty Medical Group) for many helpful discussion. We also thank Ivey Choi for manuscript editing and Eric McDonald for supporting animal study.
This research was supported by the US National Institutes of Health grant NIBIB #R01-EB-022230. This study was also supported by the Bio & Medical Technology Development Program (No. 2012M3A9C6049791), Mid-career Researcher Program (No. 2015R1A2A1A15053779) of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning, Korea.
Availability of data and materials
Please contact author for data request.
KSK mainly designed and performed all experiments, collected samples, analyzed and interpreted data, prepared the figures for the manuscript, and wrote the manuscript. SKH and HSC conceived and supervised the project, designed experiments, interpreted data and wrote the manuscript. YSK contributed to the chemical synthesis. KB and HW contributed to preparing and designing the animal experiments. All authors read and approved the final manuscript.
Ethics approval and consent to participate
Consent for publication
The manuscript has been submitted with the consent of all authors for publication.
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Small R. Botulinum toxin injection for facial wrinkles. Am Fam Physician. 2014;90:168–75.Google Scholar
- Montecucco C, Molgó J. Botulinal neurotoxins: revival of an old killer. Curr Opin Pharmacol. 2015;5:274–9.View ArticleGoogle Scholar
- Dressler D, Benecke R. Pharmacology of therapeutic botulinum toxin preparations. Disabil Rehabil. 2007;29:1761–8.View ArticleGoogle Scholar
- Peng L, Liu H, Ruan H, Tepp WH, Stoothoff WH, Brown RH, Johnson EA, Yao WD, Zhang SC, Dong M. Cytotoxicity of botulinum neurotoxins reveals a direct role of syntaxin 1 and SNAP-25 in neuron survival. Nat Commun. 2013;4:1472.View ArticleGoogle Scholar
- Coleman KR, Carruthers J. Combination therapy with BOTOX™ and fillers: the new rejuvenation paradigm. Dermatol Ther. 2006;19:177–88.View ArticleGoogle Scholar
- Carruthers J, Carruthers A. A prospective, randomized, parallel group study analyzing the effect of BTX-A (Botox) and nonanimal sourced hyaluronic acid (NASHA, Restylane) alone in combination compared with NASHA (Restylane) alone in severe glabellar rhytides in adult female subjects: treatment of severe glabellar rhytides with a hyaluronic acid derivative compared with the derivative and BTX-A. Dermatol Surg. 2003;29:802–9.Google Scholar
- Monheit GD, Coleman KM. Hyaluronic acid fillers. Dermatol Ther. 2006;19:141–50.View ArticleGoogle Scholar
- Cohen JL. Understanding, avoiding, and managing dermal filler complications. Dermatol Surg. 2008;34:S92–9.Google Scholar
- Kablik J, Monheit GD, Yu L, Chang G, Gershkovich J. Comparative physical properties of hyaluronic acid dermal fillers. Dermatol Surg. 2009;35:302–12.View ArticleGoogle Scholar
- Klein AW, Elson ML. The history of substances for soft tissue augmentation. Dermatol Surg. 2000;26:1096–105.View ArticleGoogle Scholar
- Sparavigna A, Fino P, Tenconi B, Giordan N, Amorosi V, Scuderi N. A new dermal filler made of cross-linked and auto-cross-linked hyalurnoic acid in the correction of facial aging defects. J Cosmet Dermatol. 2014;13:307–14.View ArticleGoogle Scholar
- Yeom JS, Hwang BW, Yang DJ, Shin HI, Hahn SK. Effect of osteoconductive hyaluronate hydrogels on calvarial bone regeneration. Biomater Res. 2014;18:8.View ArticleGoogle Scholar
- Chun C, Lee DY, Kim JT, Kwon MK, Kim YZ, Kim SS. Effect of molecular weight of hyaluronic acid (HA) on viscoelasticity and particle texturing feel of HA dermal biphasic fillers. Biomater. Res. 2016;20:24.View ArticleGoogle Scholar
- Wollina U, Konrad H, Petersen S. Botulinum toxin in dermatology – beyond wrinkles and sweat. J Cosmet Dermatol. 2005;4:223–7.View ArticleGoogle Scholar
- Friedman PM, Skover GR, Payonk G, Kauvar ANB, Geronemus RG. 3D in-vivo optical skin imaging for topographical quantitative assessment of non-ablative laser technology. Dermatol Surgery. 2002;28:199–204.Google Scholar
- Jacobi U, Chen M, Frankowski G, Sinkgraven R, Hund M, Rzany B, Sterry W, Lademann J. In vivo determination of skin surface topography using an optical 3D device. Skin Res Technol. 2004;10:207–14.View ArticleGoogle Scholar
- Zaheer A, Lenkinski RE, Mahmood A, Jones AG, Cantley LC, Frangioni JV. In vivo near-infrared fluorescence imaging of osteoblastic activity. Nat Biotechnol. 2001;19:1148–54.View ArticleGoogle Scholar
- Choi HS, Nasr K, Alyabyev S, Feith D, Lee JH, Kim SH, Ashitate Y, Hyun H, Patonay G, Strekowski L, Henary M, Frangioni JV. Synthesis and in vivo fate of zwitterionic near-infrared fluorophores. Angew Chem Int Ed. 2011;50:6258–63.View ArticleGoogle Scholar
- Choi HS, Gibbs SL, Lee JH, Kim SH, Ashitate Y, Liu F, Hyun H, Park GL, Xie Y, Bae S, Henary M, Frangioni JV. Targeted zwitterionic near-infrared fluorophores for improved optical imaging. Nat Biotechnol. 2013;31:148–53.View ArticleGoogle Scholar
- Kim KS, Hyun H, Yang JA, Lee MY, Kim H, Yun SH, Choi HS, Hahn SK. Bioimaging of hyaluronate-interferon α conjugate using non-interfering zwitterionic fluorophore. Biomacromolecules. 2015;16:3054–61.View ArticleGoogle Scholar
- Hyun H, Wada H, Bao K, Gravier J, Yadav Y, Laramie M, Henary M, Frangioni JV, Choi HS. Phosphonated near-infrared fluorophores for biomedical imaging of bone. Angew Chem Int Ed. 2014;53:10668–72.View ArticleGoogle Scholar
- Yeom J, Bhang SH, Kim BS, Seo MS, Hwang EJ, Cho IH, Park JK, Hahn SK. Effect of cross-linking reagents for hyaluronic acid hydrogel dermal fillers on tissue augmentation and regeneration. Bioconjug Chem. 2010;21:240–7.View ArticleGoogle Scholar
- Mckegney M, Taggart I, Grant M. The influence of crosslinking agents and diamines on the pore size, morphology and the biological stability of collagen sponges and their effect on cell penetration through the sponge matrix. J Mater Sci Mater Med. 2001;12:833–44.View ArticleGoogle Scholar
- Park KM, Yang JA, Jung H, Yeom J, Park JS, Park KH, Hoffman AS, Hahn SK, Kim K. In situ supramolecular assembly and modular modification of hyaluronic acid hydrogels for 3D cellular engineering. ACS Nano. 2012;6:2960–8.View ArticleGoogle Scholar