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  • Research article
  • Open Access

Effects of direct current electric-field using ITO plate on breast cancer cell migration

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Biomaterials Research201418:10

https://doi.org/10.1186/2055-7124-18-10

©  Kim et al.; licensee BioMed Central Ltd. 2014

  • Received: 19 June 2014
  • Accepted: 9 July 2014
  • Published:

Abstract

Background

Cell migration is an essential activity of the cells in various biological phenomena. The evidence that electrotaxis plays important roles in many physiological phenomena is accumulating. In electrotaxis, cells move with a directional tendency toward the anode or cathode under direct-current electric fields. Indium tin oxide, commonly referred to as ITO has high luminous transmittance, high infrared reflectance, good electrical conductivity, excellent substrate adherence, hardness and chemical inertness and hence, have been widely and intensively studied for many years. Because of these properties of ITO films, the electrotaxis using ITO plate was evaluated.

Results

Under the 0 V/cm condition, MDA-MB-231 migrated randomly in all directions. When 1 V/cm of dc EF was applied, cells moved toward anode. The y forward migration index was -0.046 ± 0.357 under the 0 V/cm and was 0.273 ± 0.231 under direct-current electric field of 1 V/cm. However, the migration speed of breast cancer cell was not affected by direct-current electric field using ITO plate.

Conclusions

In this study, we designed a new electrotaxis system using an ITO coated glass and observed the migration of MDA-MB-231 on direct current electric-field of the ITO glass.

Keywords

  • Cell migration
  • Electrotaxis
  • ITO plate
  • Breast cancer cell
  • MDA-MB-231

Background

In a variety of biological phenomena, cell migration plays a very important role. Cellular migrations are prominent in morphogenic processes ranging from gastrulation to development of the nervous system in embryogenesis. Migration of fibroblasts and vascular endothelial cells is essential for wound healing. In metastasis, tumor cells immigrate from the initial tumor mass into the circulatory system, which they subsequently leave and migrate into a new site. In the inflammatory response, leukocytes migrate into the areas where insult has occurred, and then they affect phagocyte and immune functions. In normal physiology and pathology, migration remains crucial for the adult organism. Finally, cell migration is crucial to technological applications in tissue engineering and playing an essential role in colonization of biomaterials scaffolding [1, 2].

Most organs (especially glands) and embryos surrounded by a layer of epithelial cells produce potential differences or transepithelial potentials (TEPs) of a few millivolts to tens of millivolts. These correspond to transcellular direct-current EFs (dcEFs) of 50-500 mV/mm, as measured in vivo or in vitro in guinea pig trachea, mouse rectum, small airways of sheep lungs and rat prostate. Endogenous EFs might also exist in the central nervous system owing to the presence of extracellular field potentials across the blood–brain barrier, including specific transendothelial [3–9].

The evidence that electrotaxis is very important in many physiological phenomena is accumulating. The cells move with a directional preference toward the cathode or anode under direct-current electric fields (dcEFs) [10–14]. The preferential direction of migration during electrotaxis varies among the cell types and under different experimental conditions.

Indium tin oxide, commonly referred to as ITO, is an n-type semiconductor with a band gap between 3.5 and 4.3 eV and a maximum charge carrier concentration in the order of 1021 cm−3[15–17]. Consequently, ITO is transparent to visible and near-infrared light and has a low electrical resistivity. ITO films have high luminous transmittance, high infrared reflectance, good electrical conductivity, excellent substrate adherence, hardness and chemical inertness and hence, have been widely and intensively studied for many years [17–20]. Because of these properties, ITO films are extensively used as coating electrodes in optoelectronic devices [21], electroluminescent devices [22], photovoltaic cells [23–25], electrochromic devices [21], liquid crystal displays [21–25], sensors [26], storage-type cathode ray tubes [21], biological devices [27], flat panel display devices and heat reflecting mirrors [28]. In this study, we designed a new electrotaxis system using an ITO glass where DC current flows on and observed the migration of MDA-MB-231 under this system.

Methods

Cell culture

MDA-MB-231 were purchased from ATCC (Rockville, MD, USA) and maintained in Dullbecco’s modification of eagle’s minimal essential medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Lonza) and 1% anti-biotics. Cells were incubated at 37°C in 5% CO2 atmosphere and the medium was changed every 2-3 days. Cells were subcultured with 0.25% trypsin/EDTA when they reach 50 ~ 70% confluence.

ITO plate electrotaxis system

Electrotaxis system using Indium-Tin-Oxide coated glasses were designed for currents contact cells directly. Indium-Tin-oxide (ITO) coated glasses were kindly provided by Kwangwoon University and were cut with approximately dimensions 2 × 2 cm2. Cu tape (3 M, USA) connected both ends of 2 × 2 cm2 cut ITO coated glasses (ITO plate) with 5 cm long electric wire, then the ITO plate was dipped into 70% ethanol for 30 minutes. The sterilized grease were pasted to one side of the silicon culture insert (Ibidi, Munchen, Germany), then put the silicon insert on the ITO coated glass surface (Figure 1A).
Figure 1
Figure 1

Indium-tin-oxide (ITO) plate electrotaxis system. Indium-tin-oxide (ITO) plate electrotaxis system (A) and Schematic diagram of electrotaxis using ITO plate (B).

Electrotaxis of MDA-MB-231on ITO plate

Fibronectin needed to be coated on the surface of ITO plate for MDA-MB-231 cell attachment. Add 50 μl of fibronectin solutions (8 μg/cm2) to silicon insert and incubate overnight at 37°C. Cells (8 × 103 cells/cm2) were seeded and allowed to grow for at least 24 hours in DMEM supplement with 10% FBS, 1% anti-biotics at 37°C in a 5% CO2 incubator (Figure 1B). Immediately before a test, medium was replaced with DMEM supplemented with 10% FBS, 1% anti-biotics. Cells were exposed to a direct-current electric field for 3 hours as indicated at 37°C in a temperature-controlled chamber on an inverted microscope stage.

Cell viability assay

Cell viability was measured using LIVE/DEAD assay kit (Invitrogen, CA, USA). After electric field treatment, cells were washed 2 times with PBS, then they treated with resazurin 5 μM and SYTOX Green 0.1 μM in dark room. Cells were incubated in CO2 incubator for 15 minutes and The ITO plate were observed by a fluorescence inverted microscope.

Time-lapse phase contrast microscopy and analysis of cell migration

We used the real time observation system (Figure 2) to observe the migration of the cells on the ITO plate. The real time observation system consisted of incubator system installed with the microscope to observe live cells migration. The incubator was regulated by temperature and gas composition controlling program (CCP ver. 3.8, Live Cell Instrument, Korea) under proper environment for cell (CO2 5%, 37°C).
Figure 2
Figure 2

Real time observation system. Schematic diagram of the real time observation system for the evaluation of cell migration.

Image acquisition

The cells were cultured in the electrotaxis incubator placed on the microscope stage, and cell images were recorded every 5 minutes until electric treatment ends by the change-coupled device (CCD) camera (Electric Biomedical Co. Ltd., Osaka, Japan) attached to the inverted microscope (Olympus Optical Co. Ktd., Tokyo, Japan). Images were conveyed directly from a frame grabber to computer storage using Tomoro image capture program and saved them as JPEG image files.

Cell tracking and evaluation of cell migration

For data analysis, captured images were imported into ImageJ (ImageJ 1.37v by W. Rusband, National Institutes of Health, Baltimore, Md). Image analysis was carried out by manual tracking and chemotaxis tool plug-in (v. 1.01, distributed by ibidi GmbH, Munchen, Germany) in ImageJ software. We obtained the datasets of XY coordinates by using manual tracking, then these datasets were imported into chemotaxis plug-in. This tool computed the cell migration speed and y forward migration index (y FMI) of cells and plotted the cell migration pathway. The migration speed was calculated as an accumulated distance of the cell divided by time. The y FMI of the cell was defined as the straight-line distance along the y axis between the start position and the end position of cell divided by accumulated distance. For each experiment, 20 cells were randomly selected along each edge of the wound. Cells undergoing division, death or migration outside the field of the view were excluded from the analysis.

Results

Viability of MDA-MB-231 on ITO plate

The appropriate strength of electric field needed to be determined before electrotaxis experiment on ITO plate. Figure 3 shows the viability of MDA-MB-231 on direct-current electric field of ITO glass. The cells under the electric field of 0 or 1 V/cm for 3 hours dyed red (Figure 3A and B). Most of cells under electric field of 2 V/cm showed little green fluorescence or slightly red fluorescence, some of them emitted bright green fluorescence (Figure 3C).
Figure 3
Figure 3

LIVE/DEAD assay of MDA-MB-231. LIVE/DEAD assay of MDA-MB-231 in an EF of 0 V/cm (A), 1 V/cm (B) and 2 V/cm (C). (Scale bar = 100 μm).

Figure 4
Figure 4

Cell tracking image of MDA-MB-231. Cell tracking image of MDA-MB-231 in an EF of 0 V/cm (A), 1 V/cm (B).

Electrotaxis of MDA-MB-231 on ITO plate

To make the electrotaxis system more convenient than the established, we considered Indium-tin-oxide coated slide glass. To identify the function of ITO glass electrotaxis system, MDA-MB-231 were seeded on the ITO plate and electrotaxis was evaluated. We confirmed the electrotaxis of MDA-MB-231 on ITO plate under direct current electric fields of 0 and 1 V/cm. In the stimulation free condition, cells migrated randomly in all directions with a scattered distribution (Figure 4A). When 1 V/cm of direct-current electric field was applied, MDA-MB-231 moved toward the anode (Figure 4B). To characterize the latency in directional cell migration, we analyzed the y forward migration index (y FMI) (Figure 5A). The y FMI value of 1 indicates the cell has migrated perfectly toward the anode, −1 means the cell has migrated perfectly toward the cathode and 0 indicates the cell has migrated perpendicular to the stimulation direction. The y FMI was -0.046 ± 0.357 under the 0 V/cm and was 0.273 ± 0.231 under dcEFs of 1 V/cm (Figure 5B). However, there were no statistical differences between the migration speed of cells under 0 V/cm (36.99 ± 9.40 μm/hr) and 1 V/cm (37.62 ± 16.23 μm/hr) (Figure 6).
Figure 5
Figure 5

The y forward migration index of MDA-MB-231. Schematic diagram of the y FMI (A), The y forward migration index of MDA-MB-231 in an EF of 0 V/cm and 1 V/cm (B) (*p < 0.05).

Figure 6
Figure 6

The migration speed of MDA-MB-231. The migration speed of MDA-MB-231 in an EF of 0 V/cm and 1 V/cm.

Discussion

The LIVE/DEAD assay kit provides two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells. The assay is based on the reduction of C12-resazurin to red-fluorescent C12-resorufin in metabolically active cells and the uptake of the cell-impermeant, green-fluorescent nucleic acid stain, SYTOX Green dye, in cells with compromised plasma membranes (usually late apoptotic and necrotic cells). The dead cells emit mostly green fluorescence whereas the healthy, metabolically active cells emit mostly red fluorescence. The injured cells have lower metabolic activity and, consequently, reduced red fluorescence emission; because they possess intact membranes, however, injured cells accumulate little SYTOX Green dye and, therefore, emit very little green fluorescence [29–31]. The more damaged cells were observed when the higher strength of direct-current electric field was applied. We chose 1 V/cm for the next experiment because most of cells were not damaged under 1 V/cm condition.

In human keratinocytes, the directionality was increased under the direct-current electric field while the migration speed was decreased [32]. This indicates that there is a difference between established electrotaxis system and ITO glass electrotaxis system, because only directionality was increased under direct-current electric field of ITO glass while migration speed was not changed. The established electrotaxis system used agar-salt bridges to applied the direct-current electric field through the media. In a new electrotaxis system using ITO glass, however, the cells contacting to ITO glass can directly be affected by direct-current electric field. To explain the directional migration of MDA-MB-231 on direct-current electric field of ITO glass and the differences between ITO glass system and existing electrotaxis system, further studies are required.

Conclusions

In conclusion, MDA-MB-231 on the ITO film coated glass in direct-current electric fields migrated toward anode. Cell viability was dependent on the strength of direct-current electric field. The migration speed of MDA-MB-231 was not affected by the direct-current electric field using ITO plate. Therefore, the direct-current electric field using ITO glass induced the directional migration of breast cancer cell. Although further studies are required to figure out the differences between established electrotaxis and ITO glass electrotaxis system, it was identified that direct-current electric field of ITO glass affected the directional migration of breast cancer cell.

Availability of supporting data

The data sets supporting the results of this article are included within the article.

Declarations

Acknowledgement

This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (Grant No. 2005-2000117).

Authors’ Affiliations

(1)
Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752, Korea
(2)
Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752, Korea

References

  1. Li S, Guan JL, Chien S: Biochemistry and biomechanics of cell mortility. Annu Rev Biomed Eng. 2005, 7: 105-150. 10.1146/annurev.bioeng.7.060804.100340.View ArticleGoogle Scholar
  2. Lauffenburger DA, Horwitz AF: Cell migration: a physically integrated molecular process. Cell. 1996, 84: 359-369. 10.1016/S0092-8674(00)81280-5.View ArticleGoogle Scholar
  3. Al-Bazzaz FJ, Gailey C: Ion transport by sheep distal airways in a miniature chamber. Am J Physiol. 2001, 281: 1028-1034.Google Scholar
  4. Dortch-Carnes J, Van Scott MR, Fedan J: Changes in smooth muscle tone during osmotic challenge in relation to epithelial bioelectric events in guinea pig isolated trachea. J Pharmacol Exp Ther. 1999, 289: 911-917.Google Scholar
  5. Wang Q, Horisberger JD, Maillard M, Brunner HR, Burnier M: Salt- and angiotensin II-dependent variations in amiloride-sensitive rectal potential difference in mice. Clin Exp Pharmacol Physiol. 2000, 27: 60-66. 10.1046/j.1440-1681.2000.03204.x.View ArticleGoogle Scholar
  6. Szatkowski M, Mycielska M, Knowles R, Kho AL, Djamgoz MB: Electrophysiological recordings from the rat prostate gland in vitro: identified single-cell and transepithelial (lumen) potentials. BJU Int. 2000, 86: 1068-1075.View ArticleGoogle Scholar
  7. Sorensen E, Olesen J, Rask-Madsen J, Rask-Andersen H: The electrical potential difference and impedance between CSF and blood in unanesthetized man. Scand J Clin Lab Invest. 1978, 38: 203-207. 10.3109/00365517809108412.View ArticleGoogle Scholar
  8. Sato MJ, Kuwayama H, Takayama ALK, Ueda M: Switching direction in electric-signal-induced cell migration by cyclic guanosine monophosphate and phosphatidylinositol signaling. Proc Natl Acad Sci U S A. 2009, 106: 6667-6672. 10.1073/pnas.0809974106.View ArticleGoogle Scholar
  9. Li J, Nandagopal S, Wu D, Lin F: Activated T lymphocytes migrate toward the cathode of DC electric fields in microfluidic devices. Lab Chip. 2011, 11: 1298-1304. 10.1039/c0lc00371a.View ArticleGoogle Scholar
  10. Robinson KR: The responses of cells to electrical fields: A review. J Cell Biol. 1985, 101: 2023-2027. 10.1083/jcb.101.6.2023.View ArticleGoogle Scholar
  11. Nuccitelli R: A role for endogenous electric fields in wound healing. Curr Top Dev Biol. 2003, 58: 1-26.View ArticleGoogle Scholar
  12. Mycielska ME, Djamgoz MB: Cellular mechanisms of direct-current electric field effects: Galvanotaxis and metastatic disease. J Cell Sci. 2004, 117: 1631-1639. 10.1242/jcs.01125.View ArticleGoogle Scholar
  13. McCaig CD, Rajnicek AM, Song B, Zhao M: Controlling cell behavior electrically: Current views and future potential. Physiol Rev. 2005, 85: 943-978. 10.1152/physrev.00020.2004.View ArticleGoogle Scholar
  14. Lin F: Lymphocyte electrotaxis in vitro and in vivo. J Immunol. 2008, 181: 2465-2471. 10.4049/jimmunol.181.4.2465.View ArticleGoogle Scholar
  15. Weijtens CHL, Van Loon PAC: Influence of annealing on the optical properties of indium tin oxide. Thin Solid Films. 1991, 196: 1-10. 10.1016/0040-6090(91)90169-X.View ArticleGoogle Scholar
  16. Nagatomo T, Maruta Y, Omoto O: Electrical and optical properties of vacuum-evaporated indium-tin oxide films with high electron mobility. Thin Solid Films. 1990, 192: 17-25. 10.1016/0040-6090(90)90475-S.View ArticleGoogle Scholar
  17. Hamberg I, Granqvist CG: Evaporated Sn‒doped In2O3 films: Basic optical properties and applications to energy‒efficient windows. J Appl Phys. 1986, 60: 123-160. 10.1063/1.337534.View ArticleGoogle Scholar
  18. Nishio K, Sei TI, Tsuchiya T: Preparation and electrical properties of ITO thin films by dip-coating process. J Mat Sci. 1996, 31: 1761-1766. 10.1007/BF00372189.View ArticleGoogle Scholar
  19. Djaoued Y, Phong VH, Badilescu S, Ashrit PV, Girouard FE, Truong V: Sol–gel-prepared ITO films for electrochromic systems. Thin Solid Films. 1997, 293: 108-112. 10.1016/S0040-6090(96)09060-8.View ArticleGoogle Scholar
  20. Mattox DM: Sol–gel derived, air-baked indium and tin oxide films. Thin Solid Films. 1991, 204: 25-32. 10.1016/0040-6090(91)90491-F.View ArticleGoogle Scholar
  21. Faughnan BW, Crandall RS: Electrochromic displays based on WO3. Topics Applied Physics. 1980, 40: 181-211. 10.1007/3540098682_12.View ArticleGoogle Scholar
  22. Meng L, Li C, Zhong G: The influence of the concentration of Er3+ ions on the characteristics of AC-electroluminescence in ZnS:ErF3 thin films. J Lumin. 1987, 39: 11-17. 10.1016/0022-2313(87)90004-4.View ArticleGoogle Scholar
  23. Bellingham JR, Mackenzie AP, Phillips WA: Precise measurements of oxygen content: Oxygen vacancies in transparent conducting indium oxide films. Appl Phys Lett. 1991, 58: 2506-2508. 10.1063/1.104858.View ArticleGoogle Scholar
  24. Valentini A, Quaranta F, Penza ME, Rizzi FR: The stability of zinc oxide electrodes fabricated by dual ion beam sputtering. J Appl Phys. 1993, 73: 1043-1045. 10.1063/1.353290.View ArticleGoogle Scholar
  25. Ozasa K, Ye T, Aoyagi Y: Deposition of thin indium oxide film and its application to selective epitaxy for in situ processing. Thin Solid Film. 1994, 246: 58-64. 10.1016/0040-6090(94)90732-3.View ArticleGoogle Scholar
  26. Luff BJ, Wilkinson JS, Perrone G: Indium tin oxide overlayered waveguides for sensor applications. Appl Opt. 1997, 36: 7066-7072. 10.1364/AO.36.007066.View ArticleGoogle Scholar
  27. Tamisier L, Caprani A: Electrochemical study of the Fe(CN)4-6/Fe(CN)3−6 couple at the ITO/NaCl interface. Electrochim Acta. 1987, 32: 1365-1369. 10.1016/0013-4686(87)85068-5.View ArticleGoogle Scholar
  28. Alam MJ, Cameron DC: Optical and electrical properties of transparent conductive ITO thin films deposited by sol–gel process. Thin Solid Films. 2000, 00: 455-459.View ArticleGoogle Scholar
  29. Visser W, Scheffers WA, Vegte WHB, Dijken JP: Oxygen requirements of yeasts. Appl Environ Microbiol. 1990, 56: 3785-3792.Google Scholar
  30. Reinheimer JA, Demkow MR: Comparison of rapid tests for assessing UHT milk sterility. J Dairy Res. 1990, 57: 239-243. 10.1017/S0022029900026856.View ArticleGoogle Scholar
  31. White MJ, DiCaprio MJ, Greenberg DA: Assessment of neuronal viability with Alamar blue in cortical and granule cell cultures. J Neurosci Methods. 1996, 70: 195-200. 10.1016/S0165-0270(96)00118-5.View ArticleGoogle Scholar
  32. Yang HY, Charles RP, Hummler E, Baines DL, Isseroff RR: The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes. J Cell Sci. 2013, 126: 1942-1951. 10.1242/jcs.113225.View ArticleGoogle Scholar

Copyright

© Kim et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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