Fig. 7

In vivo evaluations for kidney tissue regeneration and kidney function restoration properties of the scaffolds. (A) The fluorescence-based immunohistochemistry and quantitative analysis of AQP-1 expression for Native, PMEZ, PMEZ/dEV, PMEZ/IM, and PMEZ/dEV/IM 8 weeks after implantations. (Scale bars equal to 100 μm). (B) Representative H&E staining of scaffold region and quantitative analysis for assessment of regenerated glomeruli at 2 and 8 weeks after implantations (Scale bars equal to 100 μm). (C) Mouse body weight and the level of blood urea nitrogen (BUN) and creatinine in the serum at 2 and 8 weeks after implantations (n = 3). (D) CRP level at 2 and 8 weeks after implantations (n = 3). (Values are presented as mean ± SD (n = 3) and statistical significance was obtained with unpaired t tests or one-way analysis of variance (ANOVA) with Tukey’s multiple comparison post-test (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001))