Fig. 6

Expression analysis of exosomal miRNAs altered by RT in HCC and the clinical exploration and identification of candidates using bioinformatics analysis. The modulation of miR-151a-3p by radiation exposure was analyzed using qRT-PCR in the cell and exosome. (A, B). Data are shown as mean ± SEM. *P < 0.05 vs. Control by Student’s t-test. Before and after radiation exposure, exosomal miRNA was arranged into a fold change value and analyzed as a graph and ven diagram (C). Applying the GEO data set, the expression difference of each candidate miRNA between normal and cancer tissue was analyzed (D). To investigate the clinical significance of each candidate miRNA, OncomiR Cancer miRNome Atlas and patient survival were analyzed via Kaplan–Meier analysis (E). Based on the GEO data of four miRNAs in HCC tissue, the specificity and sensitivity are represented as the Area Under Curve (AUC) of the ROC curve (F)