Fig. 5

Inhibition of in vitro/ex vivo angiogenesis by treatment of RT-induced high Maspin exosomes. HUVECs were seeded on growth factor-reduced matrigel-coated plates and treated with VEGF (50 ng/mL), normal exosome (30 µg/mL), and radiation-derived exosome (30 µg/mL). After 16 h, calcein am dye (0.5 µg/mL) was added and fluorescence images were photographed using EVO M5000 Imaging System (Invitrogen) (A). Five locations of each well were randomly photographed, and the branching and tube organization were measured and compared with that of the control (B, C). Cell culture dishes were performed in at least three separate experiments. Scale bar = 200 μm. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. control by Student’s t-test; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. Normal exo by Student’s t-test. Aortas from 8-week-old C57BL/6 mice were isolated, cut into 1 mm sections, and then seeded on growth factor-reduced matrigel-coated plates. They were then treated with normal exosome (30 µg/mL) or radiation-derived exosome (30 µg/mL). After 5 days, they were stained with calcein am dye (0.5 µg/mL) and fluorescence images taken (D). Five aortic tissues of each well were randomly photographed, and the branching and tube organization were measured and compared with that of the control condition (E, F). Scale bar = 200 μm. N = 5 each, data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Control by Student’s t-test; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. Normal exo by Student’s t-test