Fig. 3

Time-dependent measurement and identification of the difference in exosome size and release amount determined by p53 gene in HCC. Electron microscopy was used to image resuspended exosome particles isolated from HepG2 (A). The expression of exosome marker proteins HSP90, CD63, CD81, and Maspin in each sample (10 µg) after radiation exposure was verified using western blot (B). Time-dependent measurements were made to determine whether the secretion of extracellular vesicles (EV) changes after radiation exposure using Nanosight's Nanoparticle Tracking Analysis (NTA) (C, D). Data are shown as mean ± SEM of three independent experiments. *P < 0.05 vs. each time of control by Student’s t-test. Based on the analyzed nanosight data, three ranges (30–150 nm, 150–300 nm, > 300 nm) were specified based on vesicle size (E, F). The secretion of EVs was analyzed qualitatively and quantitatively for the presence or absence of Tp53 genes after radiation exposure (G, H). Data are shown as mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01 vs. each time of WT by Student’s t-test