Fig. 5From: Restoration of dysregulated intestinal barrier and inflammatory regulation through synergistically ameliorating hypoxia and scavenging reactive oxygen species using ceria nanozymes in ulcerative colitisHypoxia attenuating and ROS scavenging of macrophages induced by PEG-CNPs in RAW 264.7 cells. a Fluorescence images of RAW 264.7 cells after different treatments. The hypoxia level was indicated by intracellular oxygen indicator [Ru(dpp)3]Cl2. Cells in hypoxia (1% O2) model were used as the control group. Cells in normoxia (21% O2) was used as the positive control. b Western blot analysis of HIF-1α (β-actin, loading control) in RAW 264.7 cells with different treatment strategies. c Flow cytometry analysis of CD86+ macrophages with LPS simulation. d Quantification of the percentage of CD86+ macrophages. IL-1β e, TNF-α f, IL-10 g and TGF-β1 h levels in RAW 264.7 cells with different treatment strategies. i qRT-PCR analysis of HIF-α in RAW 264.7 cells. j Representative ROS staining of RAW 264.7 cells upon different treatments using DCFH-DA probe. k Quantification of fluorescence of DCFH-DA probe (staining ROS) in RAW 264.7 cells upon different treatments. qRT-PCR analysis of IL-1β l and TNF-α m in RAW 264.7 cells. Data represent means ± s.d. n = 3. #P < 0.05, ##P < 0.01, ###P < 0.001 vs. control group; *P < 0.05, **P < 0.01, ***P < 0.001 vs. LPS groupBack to article page