Fig. 7
From: Exosomes derived from mir-214-3p overexpressing mesenchymal stem cells promote myocardial repair
miR-214OE-Exo promote migration, tube formation and apoptosis inhibition by targeting PTEN. (A) Expression level of PTEN in HUVECs after transfection with plasmids containing Vector or PTEN. (n = 3 biological replicates for each group). (B) Expression level of PTEN in HUVECs after transfection with Vector or PTEN followed by administration miR-214OE-Exo. (n = 3 biological replicates for each group). (C-D) Tube formation assays were utilized to verify the functional role of PTEN on angiogenesis in HUVECs. Scale bar = 100 μm. (n = 3 biological replicates for each group). (E-F) Scratch assays were used to validate the functional effect of PTEN on migration of HUVECs. Scale bar = 100 μm. (n = 3 biological replicates for each group). (G-H) The apoptosis rate of HUVECs transfected with plasmids containing Vector or PTEN followed by administration miR-214OE-Exo was detected by Annexin V/FITC/PI double-staining flow cytometry. (n = 3 biological replicates for each group). (I-J) The apoptosis rate of NRCMs transfected with plasmids containing Vector or PTEN followed by administration miR-214OE-Exo was detected by Annexin V/FITC/PI double-staining flow cytometry. (n = 3 biological replicates for each group)