Fig. 6
From: Exosomes derived from mir-214-3p overexpressing mesenchymal stem cells promote myocardial repair
Exosomal miR-214-3p attenuated cardiomyocyte injury via regulation of p-AKT by targeting PTEN. (A) Western blot analysis showing the level of p-AKT、AKT、Bcl2 and Bax among the different groups. (B) Neonatal rat cardiomyocytes (NRCMs) were treated with miR-214OE-Exo or miR-214OE-Exo + MK2206, then exposed to H/SD. Western blot analysis showing the level of p-AKT、AKT、Bcl2 and Bax among the different groups. (C) Quantitative analysis of the level of p-AKT、AKT、Bcl2 and Bax among the different groups. (n = 3 biological replicates for each group). (D) Quantitative analysis of the level of p-AKT、AKT、Bcl2 and Bax in Fig. 6B among the different groups. (n = 3 biological replicates for each group). (E) The predicted miR-214 targeting sequence in the 3’-UTR of PTEN. (F) Western blot analysis showing the PTEN levels after administration of Ctrl-Exo or miR-214OE-Exo in HUVECs. (n = 3 biological replicates for each group). (G) Western blot analysis of PTEN after administration of Ctrl-Exo or miR-214OE-Exo in NRCMs. (n = 3 biological replicates for each group). (H) Luciferase reporter assay was performed to confirm the target gene of miR-214. (n = 3 biological replicates for each group)