Fig. 1
From: Exosomes derived from mir-214-3p overexpressing mesenchymal stem cells promote myocardial repair
Characterization of huMSCs and exosomes which are derived from huMSCs. (A-B) Morphology and multiple differentiation potential of huMSCs. Scale bar = 100 μm. (C) Fluorescence microscopy image of lentivirus transfection in Ctrl-MSC and miR-214OE-MSCs groups. Scale bar = 20 μm. (D) The expression of miR-214-3p in cells and exosomes was verified by real-time PCR. (n = 3 biological replicates for each group). (E) Cup-shaped morphology of purified Ctrl-Exo and miR-214OE- Exo assessed by TEM. (F) NTA representative screen shot video; the bright white point represents a particle movement. (G) The particle size distribution and particle concentration were analyzed by nanoparticle tracking analysis. (H) The exosomal protein markers of TSG101, CD63, and CD81 in Ctrl-Exo and miR-214OE-Exo groups. (I) Confocal images of Dil labeled exosomes (red fluorescence) were endocytosed by HUVECs and NRCMs 6 and 24 h after incubation. Scale bar = 20 μm