Fig. 7

Ex vivo autophagy imaging in mice. A Illustration of mice model building and experiment procedure. B Fluorescence images of major organs of mice treated with only probe HFI or HFI and APAP or HFI, APAP, and NAC. C Confocal images of liver slices, corresponds to the liver of three different treatment methods, respectively. Scale bar, 20 μm. D Relative fluorescence intensity for C. E The intensity ratio of the red channel to the blue channel under different induced circumstances. Data are presented as mean ± s.d. (n = 3). **** p < 0.0001. F H&E staining of liver tissues of mice treated with HFI or HFI + APAP or HFI + APAP + NAC. Scale bar, 50 μm