Fig. 6
Fluorescence imaging of HFI in HeLa cells under rapamycin-induced autophagy conditions. A Fluorescence images of HeLa cells pretreated with HFI (10 μM) for 30 min and then incubated with rapamycin (0.1 μM or 1 μM) or rapamycin (1 μM) and 3-MA (300 μM) for another 4 h. B Relative fluorescence intensity for A. C The intensity ratio of the red channel to the blue channel under different induced circumstances. D WB illustrating the expression of LC3 II in HeLa cells. E Relative intensity for D. F Fluorescence imaging of HeLa cells co-stained with MDC (50 μM) and HFI (10 μM). Blue channel for HFI: λem = 460 ± 20 nm, λex = 405 nm. Green channel for MDC: λem = 520 ± 20 nm, λex = 405 nm. Red channel for HFI: λem = 610 ± 20 nm, λex = 405 nm. Data are presented as mean ± s.d. (n = 3). ** p < 0.01 and **** p < 0.0001. Scale bar, 10 μm