Fig. 4
Monitoring the autophagy process of HeLa cells treated with HFI under starvation conditions. A Confocal fluorescence images of HFI (10 μM) stained HeLa cells in 10% FBS-contained DMEM for 4 h, in HBSS for 0–4 h, and in HBSS and 3-MA (300 μM) for 4 h. B Relative fluorescence intensity for A. C The intensity ratio of the red channel to the blue channel at different starvation times. D Immunoblotting for LC3 I and LC3 II in HeLa cells incubated in HBSS for 0–4 h. E Relative LC3 II / LC3 I protein expressions. F Relative LC3 II / GAPDH protein expressions. Blue channel: λem = 460 ± 20 nm, λex = 405 nm. Red channel: λem = 610 ± 20 nm, λex = 405 nm. Data are presented as mean ± s.d. (n = 3). ** p < 0.01, *** p < 0.001, and **** p < 0.0001. Scale bar, 10 μm