Fig. 6

SAW exposure increased transendothelial permeability of hBMECs to chemical drugs. A hBMECs were seeded in transwell for 48 h to form firm barrier, and the in vitro BBB was exposed to SAW at 19.68 MHz at 37.8 dBm for 5 min. Then The fluorescein sodium (NaF, 376 Da, 100 μg/ml), FITC-dextran-10 kD (1 mg/ml), or FITC-dextran-70 kD (1 mg/ml) were added to the apical side (up chamber) to access the permeability of in vitro BBB. The ratio of fluorescence intensity in low chamber to up chamber was normalized with control group. B Expression of the tight junction proteins Occludin and zonula occludens 1 (ZO-1) were detected with western blotting after SAW treatment (19.68 MHz, 37.8 dBm, 5 min). Representative result of three independent experiments was shown (means ± SEM). ****, p < 0.0001