Fig. 5

Cell viability of hBMECs in SAW exposure. A hBMECs were seeded in transwell for 48 h when the firm barrier formation, then the in vitro BBB was exposed on SAW in 19.68 MHz for 5 min at 35 dBm or 37.8 dBm. TEER was measured before and after SAW treatment. B Cell viability in (A) was detected with CCK-8 kit. Results are expressed as percentage of cell viability in comparison with control. C hBMECs were untreated or exposed to SAW for 5 min at 35 dBm or 37.8 dBm. The bright field images were acquired in an inverted fluorescence microscope. Data are representative results of three individual experiments and shown as means ± SEM. Scale bar was 200 μm