Fig. 3
Assessment of murine S/R model. Methods of tPA administration included 50 μL bolus injection twice with an interval of 10 min (50 + 50) or 10 μL bolus injection and 90 μL continuous injection over 20 min (10 + 90). A Images from the dissecting microscope show normal vessel condition (pre), clot formation (60 min stroke), and thrombolysis (60 min reperfusion) in the 10 + 90 group. B Percentage of blood flow and pO2 during pre, stroke (S), and reperfusion (R). C Staining with 2% TTC shows white areas of brain infarction. The blue dotted outline marks the infarct areas. The quantified results present the percentage of the section with infarction. N = 3–5/group. Significant differences are indicated: *p < 0.05, **p < 0.01, by one-way ANOVA with Bonferroni's multiple comparisons test