Fig. 1

Methods for murine stroke/reperfusion (S/R) model establishment, treatment, and behavior assessments. A The experimental setup for establishment of the S/R model. The left photo shows the relative positions of the laser, fiberoptic probe, and mouse. The middle illustration indicates the location of a cerebral window (2.5 mm × 2.5 mm) located posterior and lateral to the bregma in the right cerebral hemispheres to uncover the anterior cerebral artery remote branch (ACArb). The right image reveals the relative locations of the focus of laser exposure and the detection point of the fiberoptic probe at ACArb. B Flow chart showing establishment of murine S/R model and OMB treatment. C, D Schematic illustration of C beam walking test and D hanging wire test for assessment of animal behavior