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Fig. 1 | Biomaterials Research

Fig. 1

From: High-glutathione mesenchymal stem cells isolated using the FreSHtracer probe enhance cartilage regeneration in a rabbit chondral defect model

Fig. 1

MSCs with high GSH levels isolated using FreSHtracer had enhanced stem cell function. A Structure of the FreSHtracer backbone and its fluorescence spectral changes upon reaction with GSH. B F510/F580 ratio from flow-cytometric analysis of MSCs according to cellular GSH levels (n = 8 each). C mRNA expression of stem cell markers in the hES-MSCs sorted in accordance with the levels of GSH, measured by real-time PCR (n = 5 per group). D (above) Transwell migration assay in hES-MSCs sorted based on GSH levels and in unsorted naïve cells (n = 5 per group). (below) Colony-forming assay in hES-MSCs sorted based on GSH levels and in unsorted naïve cells (n = 5 per group). E Evaluation of oxidant resistance of FreSHtracer-isolated hES-MSCs treated with diamide showing statistical significance when comparing GSH-high hES-MSCs to either naïve or GSH-low hES-MSCs using ANOVA. **p < 0.01, ****p < 0.0001

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Biomaterials Research

ISSN: 2055-7124

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