Fig. 8

Anti-tumor immune response induced by hydrogel. Immunofluorescence staining of (A) CRT and (B) HMGB1 in tumors after various treatments, Scale bar = 100 µm. C Representative percentages of matured DCs on day 5 after different treatments by flow cytometry. D Relative CRT level with immunofluorescence staining. E Flow cytometric examination of the intratumor infiltration of CD8⁺T cells after various treatments. F The percentage of matured DCs in lymph nodes. G The relative CD8⁺T cells frequencies in tumors with the analysis of flow cytometry. Data presented as mean ± SD (n = 5). The comparison of two groups was followed by unpaired Student’s t-test (two-tailed). The level of significance was defined as *p < 0.05, **p < 0.01