Fig. 4
Neuritogenic effect of NDs on HT-22 hippocampal neuronal cells. A Fluorescence microscope images of ND-treated HT-22 cells at 10 DIV cultured in GM, DM, and GM + ND (250-µg/mL ND-treated GM). B Quantified evaluation of HT-22 cells at each group indicating the number of neurite outgrowths, average neurite length (µm), neurofilament heavy polypeptide positive area (µm2/mm2), and the percentage of differentiation (ratio of FITC/TRITC). Scale bars denote 100 µm. Asterisks denote statistical significances compared to GM