Fig. 8

Evaluating the maintenance of tissue-specific characteristics of embedded cells within individual endometrial tissue blocks. For evaluation of whether the encapsulated cells within individual tissue blocks properly maintained their own specific cellular characteristics, block samples were incubated in specific culture media for 7 days, and the expression patterns of their typical biomarkers were assessed: endometrial stem cells were stained for estrogen and progesterone receptors (A), stromal cells were stained for cytokeratin (CK) 14 and vimentin (B), vessel cells were stained for PECAM1 and vWF (C), macrophages were stained for CD11b and CD68 (D), and myometrial cells were stained for myogenin and Myo D (E). All experiments were performed in triplicate. DAPI staining was used to label the nuclei within each field