Table 1 List of antibodies and other staining reagents
Reagents | Host | Dilution | Supplier | Cat#. |
|---|---|---|---|---|
Primary antibodies | Â | Â | Â | Â |
Anti-CD31 | Mouse | 1:200 (ICC) | Abcam | ab9498 |
Anti-collagen IV | Rabbit | 1:100 (ICC) | Abcam | ab6586 |
| Â | Â | 1:500 (WB) | Â | Â |
Anti-Laminin | Mouse | 1:100 (ICC) | Abcam | ab77175 |
| Â | Â | 1:500 (WB) | Â | Â |
Anti-VE-cadherin | Mouse | 1:200 (ICC) | Santa Cruz | sc-9989 |
| Â | Â | 1:500 (WB) | Â | Â |
Anti-β-catenin | Mouse | 1:200 (ICC) | Santa Cruz | sc-7963 |
| Â | Â | 1:500 (WB) | Â | Â |
Anti-Vinculin | Rabbit | 1:200 (ICC) | Abcam | ab155120 |
Anti-GAPDH | Rabbit | 1:500 (WB) | Abcam | ab181602 |
Secondary antibodies | Â | Â | Â | Â |
Anti-mouse-AF-555 | Goat | 1:200 | Abcam | ab150118 |
Anti-rabbit-AF-488 | Goat | 1:200 | Abcam | ab150077 |
Anti-mouse-AF-488 | Goat | 1:200 | Abcam | ab150113 |
Anti-rabbit-HRP | Goat | 1:6000 | Abcam | ab6721 |
Anti-mouse-HRP | Goat | 1:5000 | Abcam | ab205719 |
Others | Â | Â | Â | Â |
Phalloidin-AF 555 | Â | 1:500 | Abcam | ab176756 |
DAPI* | Â | 1:500 | Thermo fisher | 62,247 |