Table 1 List of the antibodies and reagents
From: Shape-memory collagen scaffold combined with hyaluronic acid for repairing intervertebral disc
Product | Catalog number | Manufacturer |
|---|---|---|
Antibody | ||
 Aggrecan (rabbit, polyclonal) | ab36861 | Abcam (Cambridge, UK) |
 Tie 2 (goat, polyclonal) | AF762 | R&D systems, USA |
 Brachyury (mouse, monoclonal) | sc-166962 | Santa Cruz, USA |
 Collagen type II (rabbit, polyclonal) | ab34712 | Abcam (Cambridge, UK) |
 Collagen type I (rabbit, monoclonal) | ab270993 | Abcam (Cambridge, UK) |
 CGRP (mouse, monoclonal) | ab81887 | Abcam (Cambridge, UK) |
 MMP-13 (rabbit, polyclonal) | ab39012 | Abcam (Cambridge, UK) |
 IL-1β (goat, polyclonal) | AF-501-NA | Novus Biologicals, USA |
 TNF-α (rabbit, polyclonal) | ab6671 | Abcam (Cambridge, UK) |
Staining | ||
 4% paraformaldehyde phosphate buffer solution | PC2031-100–00 | Biosesang, South Korea |
 Alexa Fluor® 488 secondary antibody | A11034 | Invitrogen, USA |
 Alexa Fluor® 488 secondary antibody | A11029 | Invitrogen, USA |
 Alexa Fluor® 568 secondary antibody | A10042 | Invitrogen, USA |
 Alexa Fluor® 647 secondary antibody | A21469 | Invitrogen, USA |
 DAPI | D1306 | Invitrogen, USA |