Fig. 2
From: Advanced pathophysiology mimicking lung models for accelerated drug discovery
Morphological appearance, electrical resistance, and FITC-Dextran permeability of the optimised models (static 10,000 BEAS-2B cells, static 10,000 BEAS2B-A549, CNBio BEAS2B-A549 and CNBio 10,000 A549-BEAS2B). A The morphology of cells at the end of the culture for all the models on Day 11. Scale bar represents 200 µm; B The electrical resistance of all the models over a period of 11 days where ALI was performed on Day 4; C The permeability of FITC-Dextran into the basolateral compartment of all the models. Negative (-ve) control was the medium only without FITC-Dextran. Positive (+ ve) control was empty insert without cells followed the same protocol as the inserts with cells. Mean ± SD are plotted. Statistical significance was tested using ordinary one-way ANOVA, followed by Dunn’s multiple comparison’s test for pair-wise comparisons, n = 3. D Representative IF staining of CNBio 10,000 BEAS2B-A549 and CNBio 10,000 A549-BEAS2B showing disrupted tight junctional proteins ZO-1 (red) and DAPI (blue); scale bar, 50 µm