Fig. 6

In vivo imaging of ZnPcS distribution in mice bearing U87-glioma (n = 4). a Ex vivo imaging of the major organs dissected from mice. b Ex vivo radiant efficiency of brain tumors. To show how selectively the ZnPcS enters the tumor in the brain, it is marked by dividing the radiant value in the brain into the radiant value in the liver. Quantitative data are represented as the mean ± SEM. Statistical significance is examined by students t-test (*p < 0.05 and **p < 0.01 compared with the nontreated group). c Western blot analysis of SPARC protein in the wild type and KO type U87-glioma cells. Actin is used for loading control. d Confocal images of SPARC protein and ZnPcS. ZnPcS (red fluorescence) is excited at 633 nm and monitored at 710 nm, SPARK (green fluorescence) is excited at 488 nm and monitored at 516 nm, and nucleus (blue fluorescence) is excited at 405 nm and monitored at 459 nm. Scale bar = 100 μm