Fig. 3
From: A novel DNA double-strand breaks biosensor based on fluorescence resonance energy transfer
Dynamic visualization of the DSBS in response to phosphatase activation. a Time-lapse CFP, FRET, and CFP/FRET ratio images of the WT-DSBS in HEK293T cells pre-treated with 100 μM etoposide for 30 min and then treated with 10 nM FTY720. The color scale indicates high (red) and low (blue) levels of DSBs (scale bar = 10 μm). b, c Time courses of the normalized CFP, FRET, and CFP/FRET ratio of the WT-DSBS in HEK293T cells (d, n = 1; e, n = 4;) pre-treated with 100 μM etoposide for 30 min and then treated with 10 nM FTY720. d Normalized CFP/FRET ratio of WT-DSBS in HEK293T cells pre-treated with 100 µM etoposide for 30 min and then treated with 10 nM FTY720 for 1 h before (green; n = 15) or after (purple; n = 27). All error bars represent SEM (****P < 0.0001)