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Fig. 3 | Biomaterials Research

Fig. 3

From: Magnetic transferrin nanoparticles (MTNs) assay as a novel isolation approach for exosomal biomarkers in neurological diseases

Fig. 3

Validation of exosome isolation. A Representative TEM image of the exosomes isolated from colon cancer CCM using MTNs, UC, or TEI. TEM micrographs of exosomes were revealed by immunogold CD63-labeling and CD71-labeling. Blank (no labeling) corresponds to the exosomes isolated using the three different isolation methods. B Purity of EVs (exosomes), based on the ratio of EV (exosome) particle concentration using the NTA to protein concentration measured using the Bradford assay. C Zeta potential of the exosomes isolated from colon cancer CCM using MTNs, UC, or TEI. D WB analysis of exosome-specific markers (CD9, CD81, and CD63), transferrin receptor marker (CD71), microvesicle marker (ARF6), and apoptotic-body marker (GRP78), GM130, and calnexin. Lanes 1 − 3: The exosomes were isolated using MTNs (lane 1), UC (lane 2), and TEI (lane 3).

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Biomaterials Research

ISSN: 2055-7124

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