Fig. 4

Hypoxic pretreated ADSCs secrete more cytokines and Hypo-CM promotes proliferation and migration of HUVECs. A. Heat map of the concentration of cytokines secreted by ADSCs under hypoxia and normoxia. B. Western blotting images of HIF-1α, VEGFA and β-actin from ADSCs treated with or without BAY87-2243 under hypoxia and normoxia. C. CCK-8 assay of HUVECs treated by F12, hypo-CM and nor-CM with or without cabozantinib at 24 h, and 48 h. D. Fluorescence images showing proliferation of HUVECs cultured by F12, hypo-CM and nor-CM with or without cabozantinib, respectively. (Red: Ki67, blue: DAPI). Scale bar = 100 μm. E. Representative images of the wound closure of HUVECs treated by F12, hypo-CM and nor-CM with or without cabozantinib at 0h, 12h, and 24h. Scale bar = 200 μm. F. Tube formation of HUVECs treated by F12, hypo-CM and nor-CM with or without cabozantinib at 10h. Scale bar = 50 μm. G. Statistical analysis of portion of Ki67 positive cells (fold over F12 group). H and I. Statistic alanalysis of migration area in scratch assay at 12h and 24h, respectively. (fold over F12 group). J. Statistical analysis of total branches length (fold over F12 group). The results are presented as mean ± SD (**P<0.01, ***P<0.001).