Fig. 6

Vera@CLCMP improves palmitate-induced protein aggregation. A-H HepG2 cells were treated with 500 μM palmitate or BSA (vehicle) for 12 h in the presence or absence of the release medium and incubated with 38.35 μg/mL Vera@CLCMP at 37 °C for indicated periods. A, C Triton X-100-soluble and -insoluble fractions of cell lysates were immunoblotted with anti-ubiquitin and anti-p62 antibodies. β-actin served as a loading control. B, D Band intensities were quantified and normalized to control levels. E, G Immunofluorescence staining for ubiquitin (green) and p62 (red) in HepG2 cells with indicated treatments. Nuclei were stained with DAPI (blue). Scale bar, 5 μm. F, H Sizes of ubiquitin and p62 aggregates were quantified. Data is shown as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001 (One-way ANOVA, followed by Tukey’s test)