Fig. 8

Axonal (NF-200 staining and TEM observation) and neuronal (DCX for immature neurons and NeuN for mature neurons) regeneration in lesion and peri-lesion regions 10 days and 20 days after brain-VBM placement. At 10 days, few of axons had crossed the injury boundary, and some vessels were found in the lesion area. Almost no neurons had invaded the lesion site, and a substantial number of axons and neurons were present in the graft area. At 20 days, neurons had invaded. Red dashed lines show the boundary between lesion site and host tissue. Arrowheads represent brain-VBM (with spontaneous fluorescence). V indicates vessels. Scale bars: 100 μm