Fig. 7

EX-Netrin1 improved functional recovery and reduced histopathological injury in SCI rats. (A) Representative fluorescence images showing TUNEL (green) and cleaved-caspase1 (red) double staining of spinal cord tissues. Scale bar: 20 μm. (B) The relative expression of netrin-1 mRNA in spinal cord tissues was detected by qRT-PCR. (C-D) Detection of pyroptosis-related proteins by western blot. (E-F) The PI3K/Akt/mTOR pathway-related proteins were detected by western blot. (Error bars showed means ± SD; n = 8. *P < 0.05, **P < 0.01, ***P < 0.001, vs. Sham group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, vs. Model+PBS group; ^P < 0.05, ^^P < 0.01, ^^^P < 0.001, vs. Model+EX-MSCs group; ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001, vs. Model+EX-NC group)