Fig. 6

Investigation of protein expression. The cells were incubated in the medium containing PBS, ac-LK (800 nM), Complex A (miRNA 2861, 50 nM and ac-LK, 300 nM), Complex B (miRNA 2861, 50 nM and ac-LK, 600 nM) and Complex C (Complex B and fRunx2 100 nM, ac-LK 500 nM) for 24 h. The expression level of ALP (B) and OC (C) were examined at 4, 7, 14, and 21 days through western blot. The data represents the relative value compared with PBS, control group. Quantities value was analyzed using the Prizm 5.0 software. This experiment was performed once