Fig. 4

Osteogenic differentiation property of MSCs in vitro. The cells were incubated in the medium containing PBS, ac-LK (800 nM), Complex A (miRNA 2861, 50 nM and ac-LK, 300 nM), Complex B (miRNA 2861, 50 nM and ac-LK, 600 nM) and Complex C (Complex B and fRunx2 100 nM, LK 500 nM) for 24 h. The graphs represent the quantitative analysis of alizarin red staining (A) on 7, 14, and 21 days and optical microscopic images on day 21. B Representative images of alizarin red staining showed the degree of alizarin red staining for mineral deposition on 21 days