Fig. 4
From: Self-curling 3D oriented scaffolds from fish scales for skeletal muscle regeneration
Evaluation of oriented cell growth induction cultured on DDFS. a Immunofluorescence staining of C2C12 myotubes cultured on the cell culture plate (control) or DDFSs for filamentous actin (F-actin). White arrows indicated the alignment of myotubes (scale bars: 500 μm and 100 μm, respectively). b-c The quantitative analysis of orientation angles based on F-actin staining (n = 3, mean ± SD). d SEM images of C2C12 myotubes cultured on DDFS (scale bar: 100 μm)