Fig. 1

The establishment of biomarker PTK7 in bladder cancer. A The high expression of PTK7 in BC was predicted using GEPIA, STARBASE, and GSE databases. B, C The relative expression of PTK7 mRNA and protein in BC cell lines and the normal bladder uroepithelial cell line SV-HUC-1, as assessed using qRT-PCR and western blotting. D PTK7 immunofluorescence was observed primarily in the BC cell membrane. E, F PTK7 expression was higher in BC tissues than in adjacent normal bladder urothelial tissues according to qRT-PCR and western blotting analyses. G Univariate analyses shown Kaplan–Meier curves for patients expressing low levels of PTK7 versus those expressing high levels of PTK7 in BC tumors. H Representative images of immunohistochemistry showing high expression of PTK7 in a BC tissue sample and low of PTK7 expression in a sample of adjacent normal bladder urothelial tissue. Original magnification, ⋅ 200