Fig. 3
From: Biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties
Effects of hydrogel blanket on the intrinsic properties of cells. (A) Immunofluorescence staining confocal microscopy results for Col2 and AGC expression of chondrocytes cultured for 2 days in each environment of NC, NP, HC, and HP. Results of analyzing statistical significance by quantifying the fluorescence intensities of Col2 (B) and AGC (C) in images obtained from a confocal microscope. (D) Western blot results of Col2, AGC, SOX9 genes and β-actin expressed in chondrocytes cultured in NC, NP, HC, and HP environments. Results of PCR analysis of Col2/Col1 (E), AGC (F), and SOX9 (G) expressed in chondrocytes cultured in each environment. (H) GAG/DNA amounts of chondrocytes cultured in each environment. In (B to C) and (E to F), each graph represents the mean and standard deviation, ANOVA analysis and Tukey’s post-hoc test were performed for statistical significance. N.S. (not significant), **P < 0.01, ***P < 0.001