Fig. 5

miRNA sequencing of ADSC-NVs contents and potential targets of miR-21-5p. A Highly expressed microRNAs of ADSC-NVs were identified by miRNA sequencing. B The top 10 most-enriched miRNAs in ADSC-NVs. C qRT-PCR analysis determined the expression level of the top three most-enriched miRNAs in ADSC-NVs (n = 3 per group). Results showed that miR-21-5p was the highest expressed miRNA in ADSC-NVs. Given that accumulation of miR-21 was detected in ultracentrifuged cell culture supernatant pellets and was not found in low speed centrifuged (2000 x g) pellets in a previous study [43], the high expression of miR-21-5p may be attributed to the varied molecular weight of different miRNAs. D miRTarBase analysis of the target genes of miR-21-5p. E Sequence of miR-21-5p and its predicted binding site in the 3’UTR of PTEN mRNA, and the mutant PTEN 3’UTR (MUT-PTEN). F The luciferase activity was detected in HEK-293 cells transfected with miR-21-5p mimics or control miRNA. Luciferase reporters expressing wild-type or mutant PTEN 3’UTR were used (n = 3 per group). ns, no significant difference, ****p < 0.0001