Fig. 1

Typical immunostaining showing the features of neuronal cells cultured on SCAD devices. (A) Macroscopic and microscopic images of the SCAD device. (a) Cross-section image. (b) General appearance. (c) Enlarged view of the fiber area. Scale bar = 100 μm. (B) Examples of neuronal cells cultured on SCAD device. (a) Phase contrast image of dorsal root ganglion (DRG) neurons from rat after 5 weeks of in vitro culture (5 WIV). Scale bar = 200 μm. (b) Immunofluorescence images of human induced pluripotent stem cell (hiPSC)-derived glutamatergic (green) and GABAergic (red) neurons at 6 WIV. Scale bar = 100 μm. (c) Immunofluorescence images of hiPSC-derived motor neuron spheroids at 3 WIV stained with an antimicrotubule associated protein 2 (MAP2) antibody. Scale bar = 100 μm. (d) Phase contrast image of spheroid from rat DRGs at 5 WIV. Scale bar = 200 μm. (e) Immunofluorescence images of rat DRGs at 5 WIV. Myelinated axons elongated from the DRG spheroids are stained with anti-βIII Tubulin (green) and antimyelin basic protein (MBP) antibody (red). Scale bar = 100 μm. (C) SCAD device in a multielectrode array (MEA) plate. (a) SCAD device in a Presto MEA system (MED64 Presto, Alpha Med Scientific Inc.). (b) SCAD device in a well of the MEA plate (MED-Q2430L, Alpha Med Scientific Inc.). (c) Enlarged view of the fiber area on the electrodes. Scale bar = 500 μm.