Fig. 3

The spatially confined and high-efficient transduction of PEG-treated rAAV9-hSyn::EGFP in vivo. a EGFP expression and immunohistochemical staining images of brain slices with rAAV (left) and rAAV/40% (wt/vol) PEG (right) injection at the volume of 300 nL. From top to bottom: EGFP; NeuN used to label the nuclei of mature neurons; overlay images. Scale bars, 200 μm and 50 μm. b EGFP expression and immunohistochemical staining images of brain slices with rAAV (left) and rAAV/40% PEG (right) injection at the volume of 1000 nL. From top to bottom: EGFP; NeuN used to label the nuclei of mature neurons; overlay images. Scale bars, 200 μm and 50 μm. c Transduction areas of rAAV and rAAV/40% PEG. The average transduction areas and standard deviation were calculated from three or two mice and three brain slices were selected from each mouse. **P < 0.01, ***P < 0.001, unpaired two-tailed t-test. d Transduction efficiency of rAAV and rAAV/40% PEG. The ratio between the number of EGFP⁺NeuN⁺ cells and the total number of NeuN⁺ cells within the region of EGFP⁺ cells was calculated as transduction efficiency. The average transduction efficiency and standard deviation were calculated from two mice and three brain slices from each mouse. ***P < 0.001, unpaired two-tailed t-test