Fig. 2

Biochemical and histological analysis of NRA and acellular tissues decellularized using 0.5% SDS, CHAPS, 1% TX, and combination of 0.25% SDS + 0.5% TX is shown in Fig. 2. Assessment of DNA content reveals that decellularization of tissues using 0.25% SDS + 0.5% TX eliminates cell components from native tissue to a greater extent compared to other detergents (A), Hydroxyproline content is preserved in tissues decellularized using 1% TX and 0.25% SDS + 0.5% TX (B), GAG content does not decrease significantly in tissues decellularized using 1% TX and 0.25% SDS + 0.5% TX, which suggests efficacy of these protocols in preserving ECM (C), H&E staining of native and acellular vessels (D), Masson’s trichrome staining (E), and orcein staining (F) of NRA and DRA are also shown in this Figure suggesting removal of cell components in 0.25% SDS + 0.5% TX group with little damage to ECM proteins.(* difference with NRA, P < 0.05; and *** difference with NRA, P < 0.001). NRA: Native Rat Aorta, SDS: Sodium Dodecyl Sulphate, CHAPS: 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate, TX: Triton X-100, SDS + TX: Sodium Dodecyl Sulphate + Triton X-100