Fig. 6

Tgfbr1 was directly targeted by miR-142. A The binding sequence between Tgfbr1 3’-UTR and miR-142. Mutation was generated at the complementary site of 3’-UTR and miR-142 seed region; B miR-142 mimics or inhibitor significantly changed the intra-cellular level of miR-142, and the luciferase activity of wild-type Tgfbr1 3’-UTR but not the mutant-type was significantly inhibited by miR-142 mimics. C Gene and protein level of Tgfbr1 in CDFs with different interventions; D Immunofluorescent staining of Tgfbr1 in CDFs under different stimulations. Bar = 25 μm; E The efficacy of gene knock-down of Tgfbr1 in CDFs determined by qPCR. F Migration CDFs in different groups and quantification; G The level of proteins of interest in CDFs with TGF-β intervention and Tgfbr1 knock-down determined by western blot and quantification. **: P < 0.01 compared to group NC mimics or group NC; ***: P < 0.001 compared to group NC mimics or group NC; ****: P < 0.0001 compared to group NC mimics or group NC; #: P < 0.05 compared to group NC inhibitor or group TGF-β + si-NC; ##: P < 0.01 compared to group NC inhibitor or group TGF-β + si-NC; ###: P < 0.001 compared to group NC inhibitor or group TGF-β + si-NC