Fig. 1

Plasmid map and confirmation of OCT4-30Kc19 cell-penetrating protein production. (A) Plasmid construction of pET-23a/OCT4-30Kc19 that is 69.2 kDa in size. T7, T7 tag. His, His tag. (B) Confirmation of OCT4-30Kc19 protein using coomassie blue staining and western blot analysis. Locations of band sizes, which are about 69.2 kDa were consistent in both coomassie blue staining and western blot analysis (marked with red arrowhead). Anti-OCT4-antibody (1:1000) was used for OCT4-30Kc19 detection in western blot analysis. L, protein ladder