Fig. 2

Identification of living nerve microtissues. A FDA/PI bichromatic fluorescence staining of live nerve microtissues after 1, 3, 5, 7, and 10 days of culture. Green fluorescence denotes living cells and red fluorescence denotes dead cells. B Anti-S-100 immunofluorescence staining of cells proliferating around the microtissues, showed a large number of SCs. C A general view of the chopped microtissues (1 mm ×1 mm fine particles). D The SCs count during 1, 3, 5, 7, and 10 days of culture (^**P < 0.01). SCs Schwann cells