Fig. 1From: Injection laryngoplasty of human adipose-derived stem cell spheroids with hyaluronic acid-based hydrogel improves the morphological and functional characteristics of geriatric larynxFormation of hASC-spheroids from agarose mold. A prepared agarose mold consisting of 35 cylindrical wells was placed in the wells of 24-well plates. Aliquots of 2.5 × 105 hASCs were seeded and cultured in the molds. A Aliquots of 2.5 × 105 hASCs were separated into 35 cylindrical wells of the agarose molds (scale bar: 200 μm), B and each cylindrical well-formed spheroids about 200 μm in diameter after 72 h of culture (scale bar: 200 μm)Back to article page