Fig. 4

circPRMT5 knockdown by SCNTs/si-circPRMT5 reduces the growth, invasion and migration and invasion capacity of bladder cancer cells in vitro. (A) Semi-quantitative RT-PCR was used to assess circPRMT5 expression in the SCNTs/si-circPRMT5 and other treatment groups. (B) Effects of circPRMT5 silencing by SCNTs/si-circPRMT5 on T24 bladder cancer cells proliferation, detected by CCK-8 assay. (C) Wound-healing assays to measure T24 bladder cancer cell migration (photographed at 0 and 48 h post-wounding). (D) Transwell assays T24 bladder cancer cell invasion; photographs were taken at 48 h after the cells were incubated in a Matrigel pre-coated Transwell chamber. *P < 0.05, **P < 0.01, ***P < 0.001