Fig. 11

Endometrial regeneration using cell sheet transplantation techniques in rats facilitates successful fertilization and pregnancy [113]. (A) Scheme for preparation of the full-thickness endometrium defect model and cell sheet transplantation. Isolated GFP-positive endometrial cells were separated, and two types of confluent cell cultures (stromal fraction and epithelial fraction) were harvested as contiguous cell sheets. (B) Macroscopic observations and histological analyses with H&E staining and immunostaining of endometrial cell sheets. (a–c) Endometrial epithelial cell sheet produced from syngeneic rat primary endometrial cell harvests (a), rat endometrial stromal cell sheet (b), and three-layer cell sheet assembled from one epithelial cell sheet and two stromal cell sheets (c). (d–i) Histological analysis of each cell sheet by HE staining (d–f), CK-18 (green) and vimentin (red) immunostaining (g–i). (C) Effects of endometrial cell sheet transplantation. (a–c) Macroscopic observations of resected uteri without fluorescence (a, b) and with fluorescence (c). (d, e) Histological analysis of the uterine cross section by HE staining and (f, i) GFP immunostaining. (g, h) Ultrasound image of uteri post-surgery. Reprinted with permission from Ref. [113]